FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S3_R1_D11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_D11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCATACTTAAAAAGTTAACCACAACATTATAATTACACAAATCAGAAA11.0869565217391304No Hit
GTTATCTCTATTTCCTCTTTGATCATTGATTACATTACATCCTTTAAGAA11.0869565217391304No Hit
GTCATGTCTTTCTCTTTGACAGAAGAATCGGCCACAATCATGCTCTCAAT11.0869565217391304No Hit
CAATGGAAGTTGTTCAACAAACAAGGGTAGATAAACTAACTCAAGGTCGC11.0869565217391304No Hit
GTACCAATGAACTGGCGACAGTTGAATAGAACGCCAAAATCTGGTAAATC11.0869565217391304No Hit
TTTCATCTTGATCTTGGAGGTTCCATTGGTTCTCACATATAGGAACATGC11.0869565217391304No Hit
CCTCAAGAAAGCTTCAATTATGGCGTTCACTTCTTTTCACATTTGGGAAA11.0869565217391304No Hit
CATGAGGTGAACATTGTAAGTATGGAGTTCACACTCACTGACCCGAGACA11.0869565217391304No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCATTGACCAAAATGAGAAATC11.0869565217391304No Hit
GTTCAGAAAAATCCAGAGCATGGCACCCATAATGTTCTAAAACAAAATTG11.0869565217391304No Hit
GACTAATTAACAAGACACAGAGATTTCTTTCACAATCACTGGGGACAACA11.0869565217391304No Hit
AAATAAGACAGGGACATTTCAATTCACAAGCTTTTTTTATCGCAATGGAT11.0869565217391304No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT11.0869565217391304No Hit
TCATTACTCATGTCAAACGAAGGCACGATCGCGATCGTTGACTTATCGTC11.0869565217391304No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC11.0869565217391304No Hit
GGTAGTGCTATTACTATCAGATGGAGGACCAAACTTATACAATATACGGA11.0869565217391304No Hit
CCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCT11.0869565217391304No Hit
ATGCAGCAGCGAGGCATGCCATCCACACCAATTGCCTCTTGTCAGCTGGG11.0869565217391304No Hit
GTCGCAGCTTTGCCCTATTGACAAAGTACAGATAGCCCCTAACTGCCTGG11.0869565217391304No Hit
TACCTAGAGAAAGACAACAAAATAAAATCTGAGAAGACACACAATCTCAT11.0869565217391304No Hit
AAATACTCGAATTACCACCACCTCGTGACTAGAATCTTCAATACGTAATT11.0869565217391304No Hit
CACCTACCTAAAGTTTGAACACAGTATACGATACATCGAATCAGCATCCC11.0869565217391304No Hit
GCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGAAATCAGT11.0869565217391304No Hit
TCTCAAATGAGACATAACTATTATAATCAACCCATACAGTTTTGTTTTCC11.0869565217391304No Hit
TCATAAGTCTGGCGACCTTGAGTTAGTTTATCTACCCTTGTTTGTTGAAC11.0869565217391304No Hit
GTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGACAATATACTG11.0869565217391304No Hit
ATCCCACAATGTTATACAACAAAAAGGAATTTGAACCATTTCAGTCTCTT11.0869565217391304No Hit
CTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATATACTCCAG11.0869565217391304No Hit
GTGTCCTTACGAATCCACTGTACCGGCATATGGTTGACTTAGGTACAAGA11.0869565217391304No Hit
AAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTG11.0869565217391304No Hit
ATACATAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGATGAAGGCA11.0869565217391304No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCATCCAGATATCAATC11.0869565217391304No Hit
TTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACTGGGTGAATCAA11.0869565217391304No Hit
CTAATTCCTCTATGATCATTGAATACATTACATCCTTTAAGAAATCTATT11.0869565217391304No Hit
CTGCAAGCCCATACACACAAGCAGGCAGGCAGGATTTATGTGCAACTGAT11.0869565217391304No Hit
GTTTAAAACAGATATTGCCAGTTAGGTACAGCACAATTTCCCAATACAGA11.0869565217391304No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCATCTCAG11.0869565217391304No Hit
CTTGTATTCACTCATGGATACGTGCATTCACTATGAGAGCAAAATCGTCG11.0869565217391304No Hit
AATAAATGCTGGAACAGATCATCATGATATCAGAGAACTCATCTATCTAG11.0869565217391304No Hit
GTGCCATCTATTAGAAGAGGCCTTATTAACTCAATTTTCTTCATTGTTGA11.0869565217391304No Hit
ACCTGCAAAATTTCCAAGAACTCTAAGAAGTATGGTTGCCTTCTTCAAAT11.0869565217391304No Hit
GTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAAGCAAATGTAGG11.0869565217391304No Hit
ATACTGTATAACATACGTCCGGGACCTGATATGACTCTCCCTACCTTGGA11.0869565217391304No Hit
GATGGACCCCTACATGATGGTATTGAACCAAGTCGGTATTCATAAAAAGA11.0869565217391304No Hit
CATTTACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCC11.0869565217391304No Hit
TTGCAGGTCCAAGGTAATTGTTTATCATGTTGTTCTTTATCACTGTTACT11.0869565217391304No Hit
GAGCTAGACAACAATTATCAATCCTTAAACAAGAACACAGAAAATTAGAA11.0869565217391304No Hit
CCTCAAGAGTTGGTTCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAA11.0869565217391304No Hit
GCCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTCACAAA11.0869565217391304No Hit
GGTATGTTATAAGTCTTCCTTCTTTTGTCCTACATTTGCTTATCAAAGCG11.0869565217391304No Hit
GATCATTCCCAACACATTGTCCAACGATACAATTCCCCAGTTCTGCAAAA11.0869565217391304No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGTTTAAGTGCAAC11.0869565217391304No Hit
TTATACGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG11.0869565217391304No Hit
CTTCAAACCAAAGATCCTCGACCTTGAGCAAAAGGCAATGAAAGAATATT11.0869565217391304No Hit
TCCAAGATCATTGATAAACATGTAGTTATTTATCACTAAAACTAAAATAC11.0869565217391304No Hit
CTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATT11.0869565217391304No Hit
TTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTCTGCTCTGGAGGTAGT11.0869565217391304No Hit
CTAAAGAGAAGAGCCCCTCCCAAAGAACGAAGAGTAAGCAGGCAATAAGA11.0869565217391304No Hit
AACTTAGGGGGCGTGATGTCGTCCTCAAGAATGGTTAAAATTAGGCTTTC11.0869565217391304No Hit
AGAAAGCACCATTCTGTTTTAAAGCCTGAATAGTGGATTCTTCGTAGAAG11.0869565217391304No Hit
CTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTG11.0869565217391304No Hit
GAGTTCAAGAGAATATCGCATAAATTTAATCATCCCAAAAACTAATAATT11.0869565217391304No Hit
CATTGATTCCATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCAT11.0869565217391304No Hit
ATAGATGGCCATCCGAATTCATTAGGTCGATGTCTTGCTGTCACTAAGAA11.0869565217391304No Hit
TCACAAAACCACACAGCACAGCAGACTACACCCCAGAAGTCCCTACATCA11.0869565217391304No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATCAACAAACAGGAA11.0869565217391304No Hit
CTTTCAAAGAAAAAGGAGAGTAATAGACAACATGACCAAGAAGATGGACA11.0869565217391304No Hit
GAATAATACAAATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG11.0869565217391304No Hit
GATCTTCATGATCTCAGCGAACTCTTCTTTCTTGATCCGTCCAGACTCGA11.0869565217391304No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC11.0869565217391304No Hit
GTATATAATCATGGAGTTTTAATTTTGTAAAAGATTAAGTATCGATACTG11.0869565217391304No Hit
TCATTACCCTAAGGTATATAAATCTTATTTCGAAAAGGTCGAAAGGTTGA11.0869565217391304No Hit
GTTCCTCATTTGGATTCTGTCAAAGGAATTCCACCATCCTTCCACCTCCA11.0869565217391304No Hit
GTCAAGATCTCACTTACTCTCCTTTTTCTTTGAAAGTGGCTTCTTAACTC11.0869565217391304No Hit
CCCATAATGTTCTCAAATAAAATTGCAAGACTAGGGAAAGGGTACATGTT11.0869565217391304No Hit
AGAAATAATAGATCAAGTATTGTAAAATAAATCCATTGATAGTTGCTTAC11.0869565217391304No Hit
GTTCAAGCAAATGCTGGATGAGCTTGGCACATTAGACACTGTCAAAATAA11.0869565217391304No Hit
CGATAGAAGCACTACTGGTACATCTGTTCATCATCAAGAATTCCCCTTTG11.0869565217391304No Hit
GAGAATAGCTGTTGCTCTTCTCCCAACCATTGTGAATTCTTCATACCCTT11.0869565217391304No Hit
ATCCCAATGATTTTTGCTGCAATGACGAGAGGATCACTTGAATCGCTGCA11.0869565217391304No Hit
CACAGCTGCTCTTCATACTATGATTCTAGCAGCGATAATCAAACTTTAGT11.0869565217391304No Hit
GCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCA11.0869565217391304No Hit
GCAAGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGC11.0869565217391304No Hit
GTCTATCTCAAGACTTTGAAGAACAATACAAGAGCCATCAGGGCCGTTCC11.0869565217391304No Hit
GATCTTCAGTCAATGCCCCTGCATCCTTTCCAAGAACTGAAATTCGTTTG11.0869565217391304No Hit
CCTCCGGTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGCCTCC11.0869565217391304No Hit
CAGTATGGATAACAAATAGTACCATACACAAAAAAAATACAGTGCATGTG11.0869565217391304No Hit
GTTGAACAACTTCCATTGTTTCAAGGCATGAATTCTCAAATATTCCTGGG11.0869565217391304No Hit
CTTTGACCGCTGATGGCAAAGAGGCCCATCAGGCAATCTGAGGGGGCGTG11.0869565217391304No Hit
CCTCAAGAATGCTTAAATTATGGCGTTCAATTCTTTTGACATTTCGGAAA11.0869565217391304No Hit
GCTAATAGATTTCTTAAAGGAAGTAATGGAATCAATGAACAAAGACGAAA11.0869565217391304No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA11.0869565217391304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTAT50.0145.03
TATAACA50.0145.07
GTGAGGC50.0145.0145
ATAACAT50.0145.08
GTATAAC50.0145.06
TAACATA50.0145.09
TACTGTA50.0145.02
ATACTGT50.0145.01
TGTATAA50.0145.05
CTGTATA50.0145.04
Produced by FastQC (version 0.11.5)