FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_D09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_D09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG22.631578947368421No Hit
CCTCTCAGGACAGCAGCCTCCACCCCAGATGTGCCTTCATCTGGATCTTC11.3157894736842104No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGTAAGGCAAGAG11.3157894736842104No Hit
CACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATG11.3157894736842104No Hit
GGCTTAAGTGCAACACTTCAAAATAAACACTGCCTGTTCCGCCGGCTACT11.3157894736842104No Hit
TTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTT11.3157894736842104No Hit
GATGTTGGAGACCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATC11.3157894736842104No Hit
ATTATAGGACCTGGGAGTGGGGGTAGGAAGTTGAGCACATTTTAGGAGTA11.3157894736842104No Hit
ATATTTTCAGAGACTCGAACCGTGTTAACATTACATTCAAGTCCTCCGAT11.3157894736842104No Hit
CTCCACTAGATATATCAACTAAATATAAGGAGGAGAGTAGATACAAACCC11.3157894736842104No Hit
CTGTAGAATATGTCAAATCCTGCTTGTATTCCCTCATGGTTTTTTGAATT11.3157894736842104No Hit
TGAAATAATTGACTACAAAATTAGAATCAATGTTATAGATTAAGACGAAA11.3157894736842104No Hit
TTCCAGAACAGGGCAATTGAATCCATCGCCAATGGGATTGGAATGATCGG11.3157894736842104No Hit
CTCCTATTTAAAGAACAAAGTATTTTTAAAATTTGAGTGGCTCCAGTATC11.3157894736842104No Hit
CCAATACTCATGTCAGCTGATTCATTTACTCCACACACTCCAAAGCTGGG11.3157894736842104No Hit
ATACTCCAGCAAGGAGAGAGTGGTAGTTAGTATTGAACGAGTTTTAAGGG11.3157894736842104No Hit
ATGCCAAAAGAAGTGAACGCCAAAATTGAACCATTCTTGAGGACGACACC11.3157894736842104No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.3157894736842104No Hit
TAATTGATGGCCAGCCGAATTCTGTTGGTCGCTGTCTGGCTGTCAGTAAG11.3157894736842104No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT11.3157894736842104No Hit
AAGTACTTCAAGGAATCAACAAAGAAGAAAATTGAGAAAATAAGGCCTCT11.3157894736842104No Hit
GATAAATACCAACACATAGTCGATTGAATAAATTCAACAGTTATGAAAAA11.3157894736842104No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA11.3157894736842104No Hit
GAGTACATCATCCGTAGGAAGAATACACAATGGATCGGAGAAGAGCAACA11.3157894736842104No Hit
TATGTAATCATCGCGAGGAACATTCCAGGATTTAGATTTTCAATCCACTT11.3157894736842104No Hit
GTGCTAGCAGAGCTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGAC11.3157894736842104No Hit
CAGTGTTCGTATCATAACCACACCGACGACCCCTAACCCAATAAACAAAT11.3157894736842104No Hit
GGTCATCTATCAGACAAGCACTGGAGCTAGGATGAGTCCCAATAGTTCTC11.3157894736842104No Hit
AAGTATGCTAGAGTCCCGGTATAGTTTCATTACCAACACAACGACCCCAT11.3157894736842104No Hit
CTGCTGCTCCGCTTGTCCTTTTGAAAGTGCACCCCCCAAAACTGAAAGAT11.3157894736842104No Hit
CTCCCATTCTCATCACAGATTCTCCAAGCGAATCTCTGTATATATTCAGA11.3157894736842104No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA11.3157894736842104No Hit
GATCCAAAATGGGAGACTTTGTGCGCCAATGCTTCAATCCAATGATCGTC11.3157894736842104No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT11.3157894736842104No Hit
ATCAGACAGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAGGA11.3157894736842104No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACCGCCATGG11.3157894736842104No Hit
GGAGGTGTCACTAAGCTATTCAACTCGTGCACTTGCCACTTGCATGGGCC11.3157894736842104No Hit
GTGGCGGGGTGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGCGGGGCG11.3157894736842104No Hit
ATGTGTGAGGAAGTAGTTGAACCAAGATGCATTAAGCAAAACCCAGGTAT11.3157894736842104No Hit
ATCCACACCAATTGACTCTTGTGAGCTGGGTGTACATTTGGTCTAATCAG11.3157894736842104No Hit
GTTCCTGCTGATGGATGCTCTTAAATTAAGTATTGAAGACCCGAGTCACG11.3157894736842104No Hit
ATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATAT11.3157894736842104No Hit
GAAAATCGAAACTAACAAGTTAGCAGCAATATACACACATTTGGAAGTTT11.3157894736842104No Hit
CTAGAGATCTGGGCTCTGGCTCATCACTGTCATACTGTTTAAGGTCTCCA11.3157894736842104No Hit
TAGTAGCATTGCCACAAATACATCAGAGAATGTCTGAGGACGGAGTTTAT11.3157894736842104No Hit
GTTCCAGGGTGACCATAAGTGCCTGAACAATGAGACGAAATTTAATAGAT11.3157894736842104No Hit
CTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTATAAGCGC11.3157894736842104No Hit
CAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCT11.3157894736842104No Hit
GGACGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGG11.3157894736842104No Hit
AACTAAGACCCCTGTGGACCATCGGTCCAGAATCAAAAATTACACATCAG11.3157894736842104No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTGCTGATTTG11.3157894736842104No Hit
CTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAA11.3157894736842104No Hit
CCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTATC11.3157894736842104No Hit
GATAAATTGCACTGAGTCAGTGCTAGTCAACACTTATCAATGGATAATCA11.3157894736842104No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCA11.3157894736842104No Hit
TCTTTTCATTGCCCCCTACTGGGAGCCCAGCCTGTTCAAGCTTTTCGCAA11.3157894736842104No Hit
CCCATAATGTTCTCAAACAAAATGCCAAGACTAGGGAAAGGGTACATGAT11.3157894736842104No Hit
GTATAAGTATGGTCCAACATCAGATACTAATAGCCCTACCAATGATTTGG11.3157894736842104No Hit
ATTCAAATGTGAAGAGCTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC11.3157894736842104No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTATTTGCGTGACCAT11.3157894736842104No Hit
AGAATACTGACATCAGACTCACAGCTGGCAATAACAAAACAGAAGAAAGA11.3157894736842104No Hit
CATCAGGCTGACCACTTGGCTGTTTTGTAGTAATTTGAATAGGTCTATAC11.3157894736842104No Hit
TATTTATGAATTTCTCATTTGTTTTTAATATTTTATTCTAGTTAAAGACA11.3157894736842104No Hit
AGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGAAAGGCAGAAAT11.3157894736842104No Hit
CCATGATACTACTAAGGGCTTTAACTGAGGAGGGAGCAATAGTTGGAGAA11.3157894736842104No Hit
CATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAAAGTG11.3157894736842104No Hit
ATATGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGAA11.3157894736842104No Hit
TCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAG11.3157894736842104No Hit
CTACAACTCTCTCCTTGCTGGAGTATTCATCAACTCACATTTTGCTGACT11.3157894736842104No Hit
TCCCTCCGTGTTACTCCAATTTCAATGAACCGGTTCTCTTTGTAATCATA11.3157894736842104No Hit
AATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGGA11.3157894736842104No Hit
CTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGG11.3157894736842104No Hit
CTGTAAAATAGTTCCTCCTCATGATTTCGATATGTTAAATCGGGGCAACA11.3157894736842104No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC11.3157894736842104No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC11.3157894736842104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGACA50.0145.06
TGACATC50.0145.08
CTGACAT50.0145.07
AGAATAC50.0145.01
GACATCA50.0145.09
TACTGAC50.0145.05
AATACTG50.0145.03
GAATACT50.0145.02
ATACTGA50.0145.04
GGAACAG50.0145.0145
Produced by FastQC (version 0.11.5)