FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S3_R1_D04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_D04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAGGCCTCTAGATAGAATAGCCGGGATATTCCTCAATCCTCTCGCCA11.2658227848101267No Hit
GTTACTCAAATACTCATGGCAGCTGATTCATTTTCACCAGACACTCCAAA11.2658227848101267No Hit
GGTCATTGATTATAATGTTGTTCTAAATCACTCTTACTCAAATACTCATG11.2658227848101267No Hit
TTGTTGTAATTGAATACTGGATAATTGCCTCTAACAAACTTTAGTTTTGC11.2658227848101267No Hit
GGAAAAAAGACTCCACCCCAGATGTGCCTACATCTGCATCTACAGTCAAT11.2658227848101267No Hit
TAACAACCCACATTCAAAGAAAAAGCAGAGAAAGAGACAACATGACCAAG11.2658227848101267No Hit
GGGCATTACAAACTCTTCCCCGATAATCAACATCCATTAGCACCCATTTT11.2658227848101267No Hit
GCCCCAAATAGGCCTCTAGATTGAATAGACTGGATATTACTCAATCCTGA11.2658227848101267No Hit
GTAAGCTAGAGTCCCGTTTTAGTTTAAAATTAGCCTAAAATACATATGCA11.2658227848101267No Hit
TCATCACAGGAATAAGAGATGCTGAGACATCTATTAGAAGAGGACTTATT11.2658227848101267No Hit
CCCGTATTTATGTACACACCATAAATTATATAATCAGTAGCCCTGCAGAT11.2658227848101267No Hit
GTCTTATTAATATAGGACTTCTTTTTCCTAATCTAGATACCCACAAACTA11.2658227848101267No Hit
TTAAGGAACTTGTTTTCCCAAATGAACTGGGGGCAAGAATACTGACATCA11.2658227848101267No Hit
ACCCTAAGGAATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT11.2658227848101267No Hit
GACCTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCAAAATTTCT11.2658227848101267No Hit
AAAGGAGAGTAAGAGACAACATGACCAAGAAGAAGGTCACTCAAATAACA11.2658227848101267No Hit
GCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACACAGA11.2658227848101267No Hit
TCCGTATAATGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACC11.2658227848101267No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC11.2658227848101267No Hit
TACCCTAGTCTTGCCATTTTGTTAGAGAACATTATCGGACCCATGCTGAG11.2658227848101267No Hit
ATTCAATGGTCACAAGATCCCACAATCATATACAACAAAATGGAATATGA11.2658227848101267No Hit
TATCTGTATATTTTCAGAGAATCGAACCGTGTTACCATTCCAATCAAGTC11.2658227848101267No Hit
TATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTGGAATGGATCTTGA11.2658227848101267No Hit
CTATAGAGCCTATGTACATGGATTCGAGCCGAACCGCTGCATTGACTGCA11.2658227848101267No Hit
AGTACAAACAAGGCATATTTTCATTAAGGACAAGTTAAATTCATTATTTT11.2658227848101267No Hit
ACATTAAACAGAAAGACAGCGATGAGCCATAGCCCAGATCTCTAGCAAGC11.2658227848101267No Hit
TAGTCAAACCACATGACAAAGGCATAAATCACAATTACCTCATGGCTTGG11.2658227848101267No Hit
AACTTTTAATGGAGATACTACAAACACCAATGGAAGAGGAACATGATACA11.2658227848101267No Hit
GCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGC11.2658227848101267No Hit
GGAGAAAATAAAACAACTGACAGAACTAATGTCGCATTCCCGCTCAATCG11.2658227848101267No Hit
GTCCCAATTGCTATCTCCACTTTTCATTTCTTCTCTGGAGGTAGTGCAGG11.2658227848101267No Hit
GGATAGCAAATAGTAGCATTGCCAAAACTTCTTCAGTGAATGTGTCAGGA11.2658227848101267No Hit
ATATAAACACGGCCAGTTCCGCCGGATACTGGGATAAAACATGTTTTACG11.2658227848101267No Hit
ATACTGGAGAACCTCCATACAGCCATGGAACAGGAACAGGATACACCATG11.2658227848101267No Hit
GCTGGAAATAGAAAGCTCTTAACTCTTCTTATACTTCAAGAATTAGTTGT11.2658227848101267No Hit
ACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCAAAGAC11.2658227848101267No Hit
ACTTGCCTCTCTCACCATCTTAGGTCATCCTAATTAATGTCAGTGCTCTT11.2658227848101267No Hit
CTTCAATTGTGCAACAGAACTTCATGATCTCAGAGAACTCTTCAAAATTG11.2658227848101267No Hit
GGACTTCTTTTTGCTCATGAAGATACCCACTAACTTGCAGTTCCAGTAGA11.2658227848101267No Hit
ACAATGGCCACAATTATTTCCTCAGCAATTGACTGCTCGTATCTCCCGCT11.2658227848101267No Hit
CTGTAGACACACTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC11.2658227848101267No Hit
CAATAGAGGATGATGGCAATGCAACCAAACGACTTACAGTTCTTGGAAAG11.2658227848101267No Hit
GTGGGACACTACACCTGTAAAATAGTTCCACCTCCTGCTTGCGATATGTT11.2658227848101267No Hit
CAGAATGAAGTGGATGATGGCAATGGCCCAGCATTAAGCATCAATGAACT11.2658227848101267No Hit
AATACGGCAATCGTGTTTGGATAGGGAGAACTAAAAGCATTAGTTCAAGA11.2658227848101267No Hit
GTTTCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATG11.2658227848101267No Hit
ATTTTAAGCAGACTACAGGAATGTGAAGATTCCGTATAAAGTAACTGTCT11.2658227848101267No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTCGCATCAATCCGGCCCCT11.2658227848101267No Hit
CATCACTGTCATACTGTGTAAGGTCTCCAACATCTTTGCAGTCATCAAAG11.2658227848101267No Hit
CATGAGAAAAGCATAAATCACAATTACCTCATGGCTAGGAAGCACGTACT11.2658227848101267No Hit
CAGGATCTGATTATGGAAGTTGTTTTCCCAAATGAAGTGAGGGCAAGAAA11.2658227848101267No Hit
ACATAAATACGGCCTTGCTAAATGCATCCTGTGCAGCCATGGATGACTTT11.2658227848101267No Hit
ATTCCCATCATACCAATCACATTGTCGATGGATTCAATTACCCAGTACTG11.2658227848101267No Hit
GATGACAATCCGAAATCTTTTGGTCACTCTCTGCCAGTCACTAACTAAGC11.2658227848101267No Hit
TGCCTAGGCAAAAGATAAAAGGCCATCATTGCGTGCGATTGGACCAGGCG11.2658227848101267No Hit
ATCATAGATTCCCGTTTTCCTTTAAAAATACAATTATATACAATTTCAAC11.2658227848101267No Hit
GCGGACTACACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCT11.2658227848101267No Hit
GTATGAACAATTCACAAAGGTTGGGAGAAGAGCAACACCTATTCTCAGAA11.2658227848101267No Hit
CATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAATAGGAGAA11.2658227848101267No Hit
GATGAAACGCTTGCATAAAACTTATTTCTACCAAACTATACACTCACCCT11.2658227848101267No Hit
CAATAATACGTTTGATCATACTACTCTCACAAATACACAAATTTAAATCA11.2658227848101267No Hit
ATTCAGGATATCATCCTAAAGTATATTATACCAAGAAGGTTTTTCTCTGC11.2658227848101267No Hit
GTAGAATCTGTCCACTCCTGCATGTATTCCCTAATGGTATGGTGCATACA11.2658227848101267No Hit
AGCATGAGGAGGAACTATTTTACAGCAGAAGTGTCCCACTGCAGGGCTAC11.2658227848101267No Hit
GTATACGAAACCTCTAATCTGAATCCCAGTTGTTGCGATAACCCTTCTTT11.2658227848101267No Hit
GGTTTCCGTGAAGCCGGTATCCTTGAAGCGGACCCGGCTCCTCAAAACTG11.2658227848101267No Hit
GTTCTAATTAATAAGTAACGCAATCGCCCCAAACACACAAAAAAAAATTA11.2658227848101267No Hit
GTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCATCAAGAGTTGGT11.2658227848101267No Hit
ATGGTAAGAGGCAATTCAACAGAATTCAATAACAACAAGGCAACCAAACG11.2658227848101267No Hit
GATTTATTATTTGGACAGTGACAAATGTCCCAAGCACATCCCGCATTTGC11.2658227848101267No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC11.2658227848101267No Hit
CACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAA11.2658227848101267No Hit
ATGTAATAATCTCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA11.2658227848101267No Hit
CCGGAAAACCCAATGAAAGAAAAACGGCAACATCCGAAAATCGAAACAAA11.2658227848101267No Hit
GCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGG11.2658227848101267No Hit
CAATTTAATCGAAATCTTGGCCTTTGCACCACGAATTACTTTCTTTATTA11.2658227848101267No Hit
TATGTAATCATCGCCACGAACATTCGAGGATTTTGATTTTCATTCCACTT11.2658227848101267No Hit
TTTTTAAACAATTCGACACTAAATGATGGCCATCCGAATTCTTATGGTCG11.2658227848101267No Hit
TCGCCTGCGGAAAAGGCAGTGGTAATAATGAAGTGTTACACTTAAACAAA11.2658227848101267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA50.0145.07
ATGACAA50.0145.02
TGACAAT50.0145.03
CAATCCG50.0145.06
GATGACA50.0145.01
GACAATC50.0145.04
ATCCGAA50.0145.08
TCCGAAA50.0145.09
ACAATCC50.0145.05
CCAAAAA100.072.5145
Produced by FastQC (version 0.11.5)