FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGAGACTGCGAGCTAATCTTTGCCAGCAATAACTGTTAGGCTGATAC13.8461538461538463No Hit
GCACTACGGTGGAAAGATGCATGGGGAGTAGCCAATCCGATGTCGCTACG13.8461538461538463No Hit
ATGCAATACAGCATCATGAGGTGGCGGAGGGAGTTGGGTTATCCCAGATT13.8461538461538463No Hit
GTTTTCGCCCTTCTCCTGGACTATCAGCTCTCCGCAGGCACCCAACAACA13.8461538461538463No Hit
CCTTATCCCTCCTTTGGTTCCTTGACCACAGTGGTCCTCACTCATGACAA13.8461538461538463No Hit
CCTTTGATCATCACACCAATCCATAAAGAAAGGGCAAAAATGATTAGTCT13.8461538461538463No Hit
ACACCGCACGGCAGTCTGTTGTGCTGCACACGGACAGCTGGATGCAGAGC13.8461538461538463No Hit
GTTCTTTGTTGGTATTGATATATCATAGTATATAGTTCCACAGTGTTTGG13.8461538461538463No Hit
GTTCTACCTCTTCTTCAAATCTCTTAATGATGGGCTCTTTTAAGGCTGCT13.8461538461538463No Hit
GTGAAGGCACCTGAGATTCCTGGGCAAGCGGAAGGGGCAGCATTCTGGGT13.8461538461538463No Hit
CTATTAGTCCTAACCTTATGTAAAATAGACATTTATGGGCTAACCCAGGA13.8461538461538463No Hit
TTTCTTTGTATTATATTATTTTTCTTATGTATATAAGCATGAAAATTGTA13.8461538461538463No Hit
CCTAAAATTTAATTCACAGACAATACCTGGAGTTAAAGGCGACATCACAG13.8461538461538463No Hit
AAATAAGTGATCTGAAAGCTGTACATTGTCTCATATAGGAAGATTGACAG13.8461538461538463No Hit
GTATTGGGGGGCTTCAATACGCAGTAGTGTCAGGACGGGAGCATACGGGG13.8461538461538463No Hit
CACCATTGAAAAACTAACCAGAACCCCCCAATAATCAGCAAAGCGAAAGA13.8461538461538463No Hit
CTCTTTAAGAAGTTAAAACCACAAAAAGACCTCATAACATACAGTAAGAT13.8461538461538463No Hit
AGTAGAAAATAGTAATTTAAAAATTAAAAAGATGAGTTCACAAAAACATA13.8461538461538463No Hit
CAACACACCATTCCAGTGCTCTATGGAAGCTATCGACGCCTCTGGGGATG13.8461538461538463No Hit
GAGCGAGCTGACCCGGAATGACCTGGACACCCTGCTCAGCATGGAAATCA13.8461538461538463No Hit
AAACACAACCAACAGACACCGATAAGACATGAACTCATGTGCCAACAGCA13.8461538461538463No Hit
GCACTGAACTCTGATGCTAACAAATCTGGGTCTGAATCCCAGGCAACTAT13.8461538461538463No Hit
GTTCGGTATAACGAATCTTTCAAGTATGCAGGATTTTCCACACATTTGGT13.8461538461538463No Hit
GATTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC13.8461538461538463No Hit
AGCTGATGCATGACATGGAGCACCCTCAGAGGCAGAACGTGGCTGTCAAC13.8461538461538463No Hit
AAATAGGCCAGGGGACATAGGGCAGCTTCCGAGGCATTTGGGCGTGGCTT13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)