FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_C09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_C09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGTTG22.272727272727273No Hit
CATCCACAGCACTCTTCAGTTCATGTAGATATTCATCCCTCATGGAATCA11.1363636363636365No Hit
GCTCAATGCCTCCTGTGCAGACATGCATGACTATCACCTTATCCCAATGA11.1363636363636365No Hit
CCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAAT11.1363636363636365No Hit
GGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTCATTC11.1363636363636365No Hit
CACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATG11.1363636363636365No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACCCATAAGGAAGGC11.1363636363636365No Hit
CCCTTGCCCCATTATCACATTAGCCTTCACTCATATTGAAATAATGCTCA11.1363636363636365No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11.1363636363636365No Hit
GATAAATACATTTGAAAAAAAGACTATCAGTAATCCACAGTATCATGTGC11.1363636363636365No Hit
GCCTTGTTGTTATTGAATACTGGAGAATTGCCTCTTACGAGGATCCTCAA11.1363636363636365No Hit
ATCTTCTCCTATTTCATCAACTTCTATACAGCTTGAATCAGTCAAATCAA11.1363636363636365No Hit
TCTTTCTTCTCTTTTGTTATTGACAGCTGTTACTCTGATGTCAGTATTCT11.1363636363636365No Hit
CCCATGTGAGTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGA11.1363636363636365No Hit
AGGCCCATGTTCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGAT11.1363636363636365No Hit
ACACCATAGAAGTCTTTAGATCGAATGGACTAACAGCTAATGAGTCAGGA11.1363636363636365No Hit
CAGAAGAAGGCAGCCCCTAATTTACATTCCCCATTTCATATTGATCTTGG11.1363636363636365No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCTAATG11.1363636363636365No Hit
GTACAGGTCATTACCCTAAGGTAGATAAAACTTATATCGAAAAGGTCGAA11.1363636363636365No Hit
CTCTTATACAGTCTTTGTTTTTTATTACCTATTGTTATTTGCTTGACAAT11.1363636363636365No Hit
TGTTCAACATGCTAAGTACGGTCTTGGGAGTCTCGATACTGAATCTTGGA11.1363636363636365No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGTTCAAGTTAAATTCATTATTTT11.1363636363636365No Hit
CACATATAGGAACATGTGCCTCGACACTTGGCCTATCGCAGTCCTCAAGA11.1363636363636365No Hit
GCGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAG11.1363636363636365No Hit
GTATGTGCAATATCCTCAAAGGAAAATTTCAAGCAGCTGCCCAGCGGGCA11.1363636363636365No Hit
CTCCATGGCCTCCGCTGCCTGTTCACTCGATCCAGCCATCTGTTCCATAG11.1363636363636365No Hit
CTTCTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCAGTTTTAG11.1363636363636365No Hit
ATCATGTTCCGTCCAGCCTCGAAGTCGACACTTGCAACACACCGGGACCT11.1363636363636365No Hit
CAGGTAGATATTTAAAGATGAGTCGTCTAACCGAGGTCGAAACGTACGTT11.1363636363636365No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG11.1363636363636365No Hit
TGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCG11.1363636363636365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.1363636363636365No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG11.1363636363636365No Hit
GGCCTGGACAGTGGTGAACAGTATATGTAACACCACAGGGGTAGAGAAGC11.1363636363636365No Hit
TCTTTAGAGCGAATGGCCTAACAGCTAATGAGTCAGGACGGCTAATAGAT11.1363636363636365No Hit
GTTTTAAAAGAATACCTACTTCATCTCATTCTCTTTTAAAGAAGACTCCG11.1363636363636365No Hit
GCTTACTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTG11.1363636363636365No Hit
CTGCTGGCTTCTAGCCCAGTGGCAGCGCTGCCACCTACTCTTGGGCGGGG11.1363636363636365No Hit
TATACAACAGGATGGGAACAGACACCACAGAAGCTGCTTTTGCTCTAGTC11.1363636363636365No Hit
GATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAGGGCAAGAA11.1363636363636365No Hit
GTTCCATGGGGCCAAGGACGTGACACTAAGCTATTCAGCGGGTGCACATG11.1363636363636365No Hit
GAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAG11.1363636363636365No Hit
TTTACAAACTGCTGCCCTGTGGGCTAACATGGTTCAAGAAACTGATAGTC11.1363636363636365No Hit
TAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGGAAGAGACAACATG11.1363636363636365No Hit
ACTGCTAGCCGAGAAACATCAATATTAAAAGAAATACACCTAGCAAAAGT11.1363636363636365No Hit
CCCTTGAACAATTAGCACATTAGCCTTCTCTCCTTTTTCAAGATTGCTCA11.1363636363636365No Hit
TTTTTTGACAGTATGGATAGAAAATAGTAGCAGTGCAACAACTACGTCAG11.1363636363636365No Hit
CCACCACTCCCCTGGGTGACTCTCCGATTGGCCATGTAAACGATTTGTTT11.1363636363636365No Hit
GGGTTGAGCTATTCAAGTTCCTCGAAACCAGCGAAGGGGGCAGGGTGATG11.1363636363636365No Hit
GCCCAGAGGGGAATGATGGATCTAGTAAGAGAATCTCGAACCCCAGGCCA11.1363636363636365No Hit
GCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATT11.1363636363636365No Hit
ACATGAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGCC11.1363636363636365No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTATTTTTGCTAGAGTCCCGTATTAA11.1363636363636365No Hit
GTTCATTGATGCTTAATGCTGGCCCATATCTCTTGTCTTCTTTGCCCAAA11.1363636363636365No Hit
CAGCTTATTTAACTCAAATGATCTTCTCCTCTGAATTAGTGTGACTCCCC11.1363636363636365No Hit
ATGGGAAAGAGGCCCTTCCGGCTATCTCAGGGGGCGTGGAGGCGTGCTCA11.1363636363636365No Hit
GTCTACTTTTTCTGGTGCCATATTTTCACCGAGTGCCCACTTCAATTGGC11.1363636363636365No Hit
CAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAA11.1363636363636365No Hit
TCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACC11.1363636363636365No Hit
GGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCTTCATT11.1363636363636365No Hit
ATCCAAAACCATGTCAAGCATTCAGGTAGACTGTTTCCTTTGGCATATCC11.1363636363636365No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC11.1363636363636365No Hit
ATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGC11.1363636363636365No Hit
GATTTTTGAGACCTTAAACAGTATGACATTGATGAGACAGAGCCCAGATC11.1363636363636365No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA11.1363636363636365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGCGGGGGG11.1363636363636365No Hit
CACAGAACTCTTCTATCTTGATCCCTCCAGACAAGAAGTCGACCCTGGCA11.1363636363636365No Hit
ATCTCAGAGAACTCTTATTTGATAAGTCAAGACTCGAAGGCGACCCTGTA11.1363636363636365No Hit
TTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACAC11.1363636363636365No Hit
AGTTAAAATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATATCA11.1363636363636365No Hit
CCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTT11.1363636363636365No Hit
CTACACCCTTGACGAAGAGAGCAGGGCAAGAATTAATACTAGGCTTTTCA11.1363636363636365No Hit
GATTTAGGAGTATCTAGGTGCGTATGGGGAGAGATTGTGATTCCGAGGGG11.1363636363636365No Hit
CGGCTTTTCATTTGGATCTTATTAGATCCGTGAAGAAGTACATCTGGAAT11.1363636363636365No Hit
GTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCT11.1363636363636365No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG11.1363636363636365No Hit
GCATAGAAACATGTAATAGAACCGCACCGCGGCATTGAAAAAAAGCATAA11.1363636363636365No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG11.1363636363636365No Hit
CTCCCTGGTCGGGCGGGCCCCGTTCAAATTACTCCCAGCCAGCCAAGTGG11.1363636363636365No Hit
GTTCCAGGTTGCAGGTTGTCGCTAAGTGACTGAACAATGAGCAGCAATTT11.1363636363636365No Hit
CTATCACAGTCAATGAGGATATTTGCATCCTAGTCTGTTCTTGTGGTGGG11.1363636363636365No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTAATGCACACTT11.1363636363636365No Hit
CTCACGGAATGGCTAAAGACCAGACCATTCTTGTCTCCTCTGACTAAGGG11.1363636363636365No Hit
CCTTTCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11.1363636363636365No Hit
ATTACAAAGAGAGCCGGTTCATTGAAATTGGAGTAACACGGAGGGAAGGC11.1363636363636365No Hit
GCACTAGTAGGTGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC11.1363636363636365No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGGA50.0145.08
TAAAATA50.0145.04
AAATAAG50.0145.06
AAAAGAG50.0145.0145
AAAATAA50.0145.05
AATAAGG50.0145.07
TTAAAAT50.0145.03
TAAGGAG50.0145.09
GTTAAAA50.0145.02
AGTTAAA50.0145.01
Produced by FastQC (version 0.11.5)