FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_C05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_C05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGTAAAGACAACATTGTAAGAATCTGTTCAAAGGGGGATGTGTTTG17.6923076923076925No Hit
CCCTCGCTGCCCCGGGCCTGGCGCCACACAACGGGGAAAGGTACGGTGTG17.6923076923076925No Hit
TAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACGCTGCAGTTCGCTT17.6923076923076925No Hit
CCGTAGACATATCCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTAC17.6923076923076925No Hit
GTTTGACCGTTAGCACTTCTTCTTCTGTCTTGACTGATGATCCGCATGTC17.6923076923076925No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGTGAGCAG17.6923076923076925No Hit
GCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGC17.6923076923076925No Hit
ATTGAATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTC17.6923076923076925No Hit
CCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAACAA17.6923076923076925No Hit
ATGTTGTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTAT17.6923076923076925No Hit
CTACAAGCGAATCTCTGTATATTTTCAGAGACTAGAACCGTGTTACACTT17.6923076923076925No Hit
AAAGAGAATTGGTCCGTAAAACAAGGTTTCTCCCAGTAGCCGGCGGAACA17.6923076923076925No Hit
TATTAAAAGTGTATATAATGGATGATGATTATCGTTGAAGCCTTTGTAAT17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)