FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S3_R1_B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTAACACCTTCTCAGTTCCAGGCTATAGCAGTCCATCGTTACCAC11.3513513513513513No Hit
AGATAACAAAGTCTTCCCCGATAATCATAATCCATTAGCTCCCATTTTAA11.3513513513513513No Hit
TTCAAACAACATGAGAAAGGAATAAATAGCAATTACCTCATGGCTTGTAA11.3513513513513513No Hit
CATCAGTATCATTTCTCAAATGAGACCTTCCTTTTATAATGAACCCATAC11.3513513513513513No Hit
TCTTTCAGTTTTGGTGGGTTCACTTTCAAAAGGACAAGCGGATCATCAGT11.3513513513513513No Hit
GAACTAATTAACACCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAA11.3513513513513513No Hit
CCATTGAATTTTAAAAATTTCCCAGTTCCTGATTATCCATTGATAAGTGT11.3513513513513513No Hit
ATGTAGGACCAAAGAAGGAAGACGGAAAACAAACCTGTATGGGAACATTA11.3513513513513513No Hit
CAATATAGCTGCCATGCTCAGGATCATTCTGAACCACACGGGATGATTTC11.3513513513513513No Hit
AACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAG11.3513513513513513No Hit
TATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCATCTTC11.3513513513513513No Hit
AAAAAAAAATGTTGACCAAACTATGATTAACCCACAAAGAAACATAGAAA11.3513513513513513No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTAATTTT11.3513513513513513No Hit
CACTTAACCCAAGGGACCTGCTGGGAGCAGATGTACACTCCAGGAGGAGA11.3513513513513513No Hit
CGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAG11.3513513513513513No Hit
TCTTTTGACATTTGGGAAAGCATGCCCTCAATGCAGCCGTTCGGCTCGAA11.3513513513513513No Hit
ACAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGATGC11.3513513513513513No Hit
GCCTCAATCATGCTCTCAATCTGCTGAAGAGACTGAAGAACTCAGCGCCT11.3513513513513513No Hit
AATTGTAGTAACACGGAGCGAAGTCCACATATATTACCTAGAGAAAGCAA11.3513513513513513No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11.3513513513513513No Hit
TTTAAGTACACTCCCTTAATTATGTATTCAGTAGCCCTGCAGTGGGACAC11.3513513513513513No Hit
GAAACATGCAATCAAAGCGTCATTACTTATGAAAACAACACTTGGGTAAA11.3513513513513513No Hit
GGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAGGGCAAGAATCA11.3513513513513513No Hit
GTCTACTTTGCCCAAAATGACAAATCCTCTCAGGACAGCAGACTACACCC11.3513513513513513No Hit
ATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA11.3513513513513513No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT11.3513513513513513No Hit
CATTCACATCATTTCATTCACTCGAGAGGAGATGGCCACCAACTCACAGA11.3513513513513513No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT11.3513513513513513No Hit
ACTTCCATAATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCC11.3513513513513513No Hit
CACTATTTCCTCTTTGTTCATATATTCGATTACATCATTTAAGAAATCAA11.3513513513513513No Hit
GGCATTACCACAGCATTGTTTACAGAATCAATCACTTTAAGACTGACAAA11.3513513513513513No Hit
CTTTTGAAGCACCGATTTGAGATAATTGAAGGAAGAGCCGGAATCATGTC11.3513513513513513No Hit
CAGAAAGAACTGGATGATGCCAATGAGATACCCAAGTACAGCAGACAAGA11.3513513513513513No Hit
CTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAA11.3513513513513513No Hit
GAATGAAGTGGATATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTG11.3513513513513513No Hit
CCCCAACTCAACCCGATTGATGGACCACAACCTGAGGATAATGAACCAAG11.3513513513513513No Hit
CGAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAATTTTGATTATCGC11.3513513513513513No Hit
GTCTTCCCCGATAATCATCCTCCATTAGCTCCCATTTTAAGCAGACGTCA11.3513513513513513No Hit
CTACAGGTACATCTGTTCATCCACAACAATTCCCCTAACCCTTGTGTTGA11.3513513513513513No Hit
TACTGCAGGGATCCGCTACAATCAACCGACGATTTTGCACTCATAGTGAA11.3513513513513513No Hit
CTAATAGCCCTACCTTTGATTGGGATTGATCCCACAGCTTCTTTAAATCA11.3513513513513513No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAATGGTT11.3513513513513513No Hit
CGAGAAGAACATATGAGTTAAAGAACCTCTGGCATCAAACCCAATGAAAG11.3513513513513513No Hit
ATTCACAGTCAATGAGGAAAATTGCATCCTACTCTCTTCTGGTCGGGCAG11.3513513513513513No Hit
GGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTCTCATA11.3513513513513513No Hit
AGTTTCAACAAAGTATACGAAACCACTAAACTGCATCCCAGGTGTTTCGA11.3513513513513513No Hit
GAAGTGTCCAACTGCAGGGATACAAAATACATAATCAAAGGAGTGAACAT11.3513513513513513No Hit
GATTCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCT11.3513513513513513No Hit
CCACATATATTACCTAGAGAAAGGCAACAAAATTAATTCTGAGAAGACAC11.3513513513513513No Hit
CTAGAGTCCCGGTTTACTTTTATTAGCAAAAATACGTCCCCTTGGCCCAT11.3513513513513513No Hit
AAGTACGTTCCCTCTTAGATCTCTAACAATTAAAAATCGGTCAATACTCA11.3513513513513513No Hit
TTGAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11.3513513513513513No Hit
CTAGACACCATGGACTCCACCAGGCTAGAAATTACAACCGGTCTCCTAGA11.3513513513513513No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCATT11.3513513513513513No Hit
CCTATATGAGGATCTGTAGTCTTTGATCAACAATTGAATAGCCATCTGCT11.3513513513513513No Hit
CTTCAATTGTCACTTCGCCTCTTTCGGACTGCCGAAAGGAATCCCAAAGA11.3513513513513513No Hit
AGCCAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAAGTAGA11.3513513513513513No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG11.3513513513513513No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC11.3513513513513513No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTAGTGCGACAATGCTTCAATCC11.3513513513513513No Hit
GGCCTCCACCATGCTATAAATTCCAACCGGTCTACTATATGAACTGCTAG11.3513513513513513No Hit
GATAATAACCAAAGCTTCGGTCTGTAAGACAATTGGAATGGCTAACTAAA11.3513513513513513No Hit
GACTGGAAAGAGTCTTTCCAGGAAAGAATACACATCTTGAGGCTCTCATG11.3513513513513513No Hit
ATACAGAATTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCC11.3513513513513513No Hit
ATGTTGAACATTCCCATAATCATCCCACGACTCAGTGATGCAGTGCAATC11.3513513513513513No Hit
AGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGAT11.3513513513513513No Hit
CGCCCATCAGGCAATCTGAGGGGGCGTGGTCTCGTCCTCAAGAATGGTTA11.3513513513513513No Hit
ATGCTGGATCAGACCCAGTGAAGGTATCACCTCTGGCCGTAACATGGTGC11.3513513513513513No Hit
TGATTACAAATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTG11.3513513513513513No Hit
GACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTT11.3513513513513513No Hit
GACCATCGCTGGAGCTAGCATGAGTCCCAATAGTTCTCATTGCATGTACC11.3513513513513513No Hit
CTTCTAAAAGATCGCACAGCATCACTGAGTCCAGGGATGATAATGGGCAT11.3513513513513513No Hit
ACCTTTGATTGGGTTTGATACAACAGCTTCTTTAAATCAAATAATCTTCT11.3513513513513513No Hit
GTATGACAGTAATGACACAGAACCCAGATCTCAAGCAAGCTGCGTACAAA11.3513513513513513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCA50.0145.05
TTCACAG50.0145.02
CAGTCAA50.0145.06
GTCAATG50.0145.08
TCAATGA50.0145.09
ACGGCTT50.0145.0145
TCACAGT50.0145.03
CACAGTC50.0145.04
ATTCACA50.0145.01
AGTCAAT50.0145.07
Produced by FastQC (version 0.11.5)