FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAGGATCATGACCTGAGCCAAAGGCAGATGCTTAACCAACTGAGC19.090909090909092No Hit
TGTAGGCCCACACATGGTATCGGTAATGTGATCTAAATACTACTCTCCAG19.090909090909092No Hit
GTCTTTCTCTTTGACAGAAGACTCGGCCTCAATCATGCTCTCAATCTGCT19.090909090909092No Hit
CTTCAATATAAACACTGCCTGTTCCGCCGGCTACTCGGAGAAACCTAGTT19.090909090909092No Hit
AAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACCGTGGTGAACTCC19.090909090909092No Hit
CATGTGGTTTGACTATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATG19.090909090909092No Hit
CTAAGTGAATGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACAC19.090909090909092No Hit
GAACAAAATGATAAACAATGACATTGGCCCTGCAACGGCCCAGATGGCTC19.090909090909092No Hit
CACATATATGAGGACCGAACCGGACGTAAGAACGGAGGTTTATGGACAGA19.090909090909092No Hit
CTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTC19.090909090909092No Hit
GAACCTATAATGGTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTAAAT19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)