FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAATTATAGAAGATACAATCACCCAAAACATACTGAAAAAACAAAAAT15.263157894736842No Hit
GTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGATTGAACAGTCTGTGCT15.263157894736842No Hit
CTGAAAATATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGGGA15.263157894736842No Hit
CCCTTGATCCTCTTCATTCATCGAAGCCCTTGCATCCTTTCAAATAACAG15.263157894736842No Hit
ACTCAGCACCGTCTGGCCAAGACCACCCCACAGTGGCACTGTTTACCCCA15.263157894736842No Hit
CGATTGAACATAACCCAAGCATGACCAGGAAATATTAAACAGCAGAAGTG15.263157894736842No Hit
CTAATTCTATTGTACAATATTCAGTATCGATACTCACAAGACACTAATTA15.263157894736842No Hit
GAAATTGTATGACAAAATAAGGAAGACTGAAAACAAGCCGGGAGGGGGTC15.263157894736842No Hit
GTACAGGTCATTAAACTAAGTGATATAAAACTGCGGTAGAACATGTCTAA15.263157894736842No Hit
CCCCAGCTCAACACGATTGATGGACCACTACCTGAGGATAATGAACCAAG15.263157894736842No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA15.263157894736842No Hit
AAAGTGCTTTTACAGACCTGGGGAATTGAATCCATCGACAATGTNATGGG15.263157894736842No Hit
GACTGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15.263157894736842No Hit
GCCATGATCATGCAATCCTCCTGTGATAATACCATCCACAAAATTATTGA15.263157894736842No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA15.263157894736842No Hit
TGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGATTA15.263157894736842No Hit
GGTATTTGTGTTCGAATCTTCATAATTTTACTCTCGAAAATGTACACTAT15.263157894736842No Hit
GACACAGTAAACAGAGCACACCAATACTAAGAAAAGGGAAAGTGTACTAC15.263157894736842No Hit
AGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAA15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)