FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R1_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R1_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTACTGTGCACCACGAGTACTGACTAAACAACAAGTTCTCCTTACTA14.545454545454546No Hit
AAATAGGAGACATGCTCTTGAGGACTGCGATAGGCCCAGTGTCGAGGCCC14.545454545454546No Hit
GGATAAGAGTCAGAAAAATGGGAGTATATTAATACTCCAGCACTGATAGA14.545454545454546No Hit
AGTAGAAACAAGGTAGTTTTTTACTATAGCTCTATGTTGACAAAATGACC14.545454545454546No Hit
TCCTATTTCAAGAACACAGTAGTTTTCCCATTTGTGTGGCTCCAGTCTCG14.545454545454546No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACA14.545454545454546No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA14.545454545454546No Hit
GTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTT14.545454545454546No Hit
ATTTCATCTTGATCTTGGAGGGTCCATTGGTTCTGACATATATGCACATG14.545454545454546No Hit
GTATGGGTTCATTATAAAATGAAGGTCTCATTTGAGAAATGATAATGATG14.545454545454546No Hit
GTATCTACTTCGCGCTCCCTTTCTGACATGACCCTAGCGGAAATGTCACG14.545454545454546No Hit
AGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTGTTCAC14.545454545454546No Hit
GCTTGGAAGCAGGTGCTAGCACAGCTACAGGACATAGAAAATGAAGAGAA14.545454545454546No Hit
ACTTCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCATCGTTTGTTTT14.545454545454546No Hit
GTATTAAGTATCCCTGCAGTGGTACAATTCTTATTTAAAATATTTCATAA14.545454545454546No Hit
ATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTG14.545454545454546No Hit
GGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCT14.545454545454546No Hit
CCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGG14.545454545454546No Hit
CATATATACTGTTGAATACAGATTTTGCCAGTAAGTTCCTGCACACTTTC14.545454545454546No Hit
ATACTGCCTCTTGTAAAAAATAAATAAACCAAGACAATATACTGGTTGGC14.545454545454546No Hit
TCCAAGGACTGTAAGTCGTGTCGTTGCCTTGTAGTAATTGAAAACAGGAG14.545454545454546No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCC14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)