FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_H09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_H09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502274
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC16080.32014398515551273No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT15300.3046146127412528No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC12450.24787267507376454No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT10150.2020809359035108No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC9850.19610810035956472No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC9610.19132983192440778No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG9500.18913979222496088No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC9070.18057872794530477No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA9060.18037963342717322No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG8850.1761986485464109No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA8700.17321223077443787No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG8060.16047018161401944No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC7420.14772813245360103No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT6870.13677793395636645No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA6740.13418970522065646No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC6440.1282168696767103No Hit
GAAACAAGGAGGGGAACCAAAGGTCGAGGTGGCAGAGGGGAAATGAATGG6430.1280177751585788No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA6320.12582773545913187No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG5950.11846123828826496No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG5930.1180630492520019No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC5780.11507663148002883No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT5750.11447934792563422No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA5620.11189111918992421No Hit
GTCTGGGAGAGCCTAAACGCCCGGAGCCCAGCAGGTGCTTTAACGCGAGA5480.10910379593608269No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT5260.10472371653718886No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT5240.10432552750092579No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA5140.10233458231961041No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT5050.10054273165642656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1251.8189894E-1257.984621
CCTAACG400.00604833154.376839
TAAGATG5350.052.8364525
CTCGACA604.939465E-448.339775145
GCATTAA6000.045.9044951
GCGAAAG1602.0008883E-1145.2914662
AGATGTA6150.044.7937937
GTAGAAA5700.041.95422
CATTAAG7000.040.3741042
CGAAAGC1807.2759576E-1140.2670973
TAGGACG907.488805E-540.2670974
ATGAACG1105.265043E-639.538916
AGTAGAA5750.039.076591
TTAAGAT6750.037.582634
ATTAAGA7550.035.5203823
TAGAAAC6800.035.1744963
CGGATAT1051.8520374E-434.5249759
ACGGATA1051.8520374E-434.5249758
GTATATA3500.033.134071
AAGATGT8800.032.9490936
Produced by FastQC (version 0.11.5)