FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_H08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_H08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460343
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC68480.4689309292405962No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG53980.36963918750594893No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG47610.3260192982059694No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC39420.26993658339171No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT39240.2687039962529351No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG31650.21672990523459215No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA29400.2013225659999055No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT27970.19153034595297133No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC22980.1573602913835996No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG22570.15455273178972337No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC22570.15455273178972337No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC21870.14975933736115418No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT21550.1475680713366654No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG19710.134968291695855No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA18790.1286684018754498No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC18360.125723888155043No Hit
GGATTAGGTAGGAAGAGACTTGGAACATAGAAACTTAAAGAAAATCTTGG18200.12462825514279864No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG17880.12243698911830987No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA17840.12216308086524878No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA17700.12120440197953494No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT17470.11962942952443364No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG17280.11832836532239344No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA16630.11387735621015062No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC16150.11059045717341748No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC16130.11045350304688692No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT16120.11038502598362165No Hit
GGTAGGAAGAGACTTGGAACATAGAAACTTAAAGAAAATCTTGGCCAAAG16010.10963177828770365No Hit
ATGTTTATTCACTCGAGAGAAAACACTTGCACATCACAGCTTAGCGTTCT15500.10613944806117466No Hit
GTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTACAAGCTGTAATCA15260.1044959985428081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAC11850.095.3410953
GCAGGTA12250.091.033032
GGTACTG12650.089.911855
AGTAGAA6750.082.598391
AGCGAAA3100.079.414881
ACTGATC15650.074.5484168
AGGTACT16700.071.133744
CGAAAGC3750.065.6631243
GTACTGA18500.063.046646
GCGAAAG3950.062.3298722
GTAGAAA9050.061.6107522
GACAGTA13700.060.2929427
TACCGAT502.0225688E-457.930112
ACCGTCA400.00606966954.333676
GTACCGA400.00608202154.305761
AGCAGGT21500.051.864111
CAATTCG3900.048.3098459
TTAGGTA5700.048.2899784
ATGTAAC8500.047.7284055
GTATATG9350.046.4648171
Produced by FastQC (version 0.11.5)