FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738922
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC9670.13086631606583646No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA9450.12788900587612767No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT9160.12396436971696606No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA9090.12301704374751328No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC8890.12031039812050529No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA8800.11909240758835168No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC8130.1100251447378749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2950.071.199681
GCGAAAG3400.061.772012
AGTAGAA6800.055.385551
CGAAAGC3800.055.2884063
CGCGAAC700.001055341441.4375145
GTAGAAA8900.040.6867142
TAGAAAC10200.036.223443
CCTTATA5400.033.5288662
CCCTTAT6650.031.5848181
GCGATTA950.00472152130.532896145
TTATACT6900.030.452814
CAATTCG1504.4049357E-528.9924939
GAAACAA13050.028.3164065
GTATACC1306.504016E-427.8679564
CGGTTCG1050.00774501427.5913371
TATACTG7400.027.4160465
GTATATA1606.872566E-527.1602211
TATACGT1658.4499196E-526.3478854
GTACATA2207.547351E-726.3371851
ATGAACG1701.0346529E-425.5746826
Produced by FastQC (version 0.11.5)