FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224971
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4250.040.912551
CGAAAGC6400.029.4289262
ATCGCGC1000.006082411428.9927468
TATAATA6900.028.3462542
GATCGCG1100.00968807626.3548877
TACGCTG1100.009695793526.3505825
ATATACT6450.024.7134084
GCGAAAG7650.024.6232951
GTATTAC9400.023.8928551
GTATATG4550.023.8843861
TACATGC7600.022.887147
GTATATA10600.022.5549741
TATATAC10950.022.493843
ATATAAC6200.022.2003963
TATACAG6350.019.399845
TTAGTAC7850.019.379763
ACATGCC8800.018.9441228
GTATTAG5801.0913936E-1118.7368891
ATAGTAC5801.0913936E-1118.735363
TAGTACA10450.018.720494
Produced by FastQC (version 0.11.5)