FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_H01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_H01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765444
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2450.056.1204951
CAATTCG1604.6038622E-836.2350469
GTTACGC1952.6349335E-729.7060473
CGGTTAT1255.208857E-428.946361
GTATAGA4750.028.946361
TTACGCA1804.7396297E-628.1625394
GCGAAAG5250.027.5715662
TATACAG6200.026.868245
CGAAAGC5550.026.093153
TACGCAG2101.5665473E-524.1424775
TAATACC3601.8735591E-1024.1393184
TATATAC8400.024.136163
GTATAAT2101.5768932E-524.1219651
TATAGAC3753.1468517E-1023.1601142
GTATTAT4455.456968E-1222.76681
TGTATAT5750.022.6536731
TAGTACG1600.002189041322.6306134
TATATAT5200.022.2693422
CAGCTCG6400.021.5145599
TGTAATA4851.6370905E-1120.8918572
Produced by FastQC (version 0.11.5)