FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_G06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_G06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885753
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG3300.061.4995469
AGCGAAA2250.051.5337451
TATACAG3950.036.693335
GTATAGG3100.032.7280851
CGAAAGC3950.029.3480342
TATATAC8000.028.9860943
GCGAAAG4050.028.6298561
GTCTAAG3351.8189894E-1228.1224251
TATACCA2105.00384E-727.607365
TCTACGA1050.007732437527.6011242
ACAATTC7800.026.948298
CGTATAC1358.104651E-426.8389743
GCTATAC1658.4450054E-526.3509943
GTATATA8000.026.2641942
ATTCGAC4200.025.88191
TTTAAAC8150.025.7851733
TATATTC4051.8189894E-1225.0497113
CAGCTCG5800.024.9936569
TATAGTA2101.5626421E-524.1509842
ATATACA7300.022.831514
Produced by FastQC (version 0.11.5)