FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981054
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT15880.16186672700993013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2600.091.911521
CGAAAGC3650.065.4912643
GTACGGC502.0188387E-457.949843
GTATTAC5800.044.947331
CTAATAC3550.038.769263
TATTACA7200.037.220932
GGATAGC1051.8664908E-434.4834021
TATAGAC8550.033.0382082
ATTACAT8700.031.6392823
CCTAATA3700.031.3210452
ATATAAC2401.5970727E-930.1822093
GTTATAG1204.085928E-430.1729741
GCGAAAG8550.028.8025422
GTATTAG4700.027.733461
TTACATC9450.026.8327374
ATAGACC7850.026.7602793
TACATCA9700.026.1451745
CCCTTAC1958.834202E-625.9951781
GTAAGAC3357.6397555E-1125.9476933
TGGCCCG1400.001002709825.8783875
Produced by FastQC (version 0.11.5)