FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421798
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20020.1408076252744764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3800.061.001911
CAATTCG6850.050.785739
CGAAAGC6700.034.6054043
GCGAAAG6550.034.2796442
TTATACT7500.028.9830444
GGATAGC7600.028.6037035
TAACGCG1306.5038353E-427.8702745
TATATAC9100.025.4787033
TTTAAAC16400.024.2989523
GATAGCA9400.023.127216
TAGAAAC12900.023.0202522
TATACTT10750.022.2443665
ACAATTC16050.022.1256898
ATATTCA9550.022.0082579
TATATTC9700.021.6671628
GTTACGC3355.5391865E-821.6283763
CCTATAC4409.822543E-1121.4071773
GTTATAC4409.822543E-1121.4071773
TTAACGC1700.00310393921.3110624
CTATAAG5450.021.266721
Produced by FastQC (version 0.11.5)