FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613667
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT11120.18120576794906684No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA9320.1518738990364481No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC8260.13460068734346153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2050.053.015851
GAGCGAA1807.2759576E-1140.2527771
GTATTAC5500.036.8861771
CGAAAGC3150.034.4939463
ATTACAT6300.032.194353
TATTACA6800.030.8898532
TTCGGGT1255.177706E-428.9749134
TATAGAC6750.027.8994942
TACATCA6850.027.4944445
GTATTAT2405.2965333E-827.1706241
ATATACT4300.026.9534074
GTTCGGG1358.120077E-426.8286233
GCGAAAG4050.026.8264372
TGTTCGG1358.123944E-426.8264372
GTATACA1907.1840986E-626.6939471
TTACATC7600.025.7342974
TAGTATG1801.52492E-424.1536357
TTTACGG1600.002186196422.6348062
TAGACAT8050.022.496054
TATATAC6450.022.4611743
Produced by FastQC (version 0.11.5)