FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_F03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_F03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677855
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG32310.19256729574367273No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30820.18368690977468255No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30260.18034931504808221No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA26800.15972774763015873No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC26050.15525775469274758No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA23350.13916578011806743No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA23060.1374373828489351No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT22730.1354705859564742No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT22540.13433818774566336No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC22380.13338458925234897No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC21670.12915299593826643No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG20720.12349100488421229No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG20180.12027260996927625No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC19030.11341862079857915No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT18530.1104386255069717No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG18140.10811422917951789No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG17400.10370383614793889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2300.078.757981
CGACTCG400.00604141154.39816145
CAATTCG5050.048.7962579
AGTAGAA10750.043.1373981
CCTTATA9850.042.6537932
TTATACT11550.039.5174754
CGAAAGC4800.039.2407073
AGGTCGT3600.036.229756
GTAGAAA13000.036.218952
CCCTTAT13200.034.5819131
GCGAAAG5450.033.2283942
GACAGTA3750.032.852237
GTCTAAG12300.031.8105411
GGTCGTT4250.030.692397
ACAATTC8650.030.1619538
CTAAGGA14300.028.8764323
TCTAAGG13600.028.7621082
CTGCGTA1100.0096885226.3551989
CTTAGAC13850.026.1532043
TCGTTTT5000.026.0916449
Produced by FastQC (version 0.11.5)