FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_F02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_F02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895279
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATACTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAG26090.29141753576259466No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT25870.2889602012333585No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA14730.16452971643476502No Hit
CTATGTGTACTGCATAACAAACCGCTCCCACCTAACTAAGGAATTTCAAA14170.1582746830876185No Hit
ACACTGGGGATCCCACACTTGGCCGTTATGGGAGGCCCGGCTAGAGTCAG11220.125324060991043No Hit
GTATACTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAGCT11080.12376030265425639No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC10430.11649999609060414No Hit
GCTTCAATATCCCCATGCAGTTAAAAATATACAACATTGAAGATCGAACA10120.11303738834486234No Hit
GCATAACAAACCGCTCCCACCTAACTAAGGAATTTCAAAAAGAGTCCCTC9770.10912799250289575No Hit
GGGCTATACCTTCGTCATTCCCTCAGGCTGGATTCATGCTGTGTATACTC9620.10745253714205293No Hit
TTCTAGCACTGGTGAGGCTTGCAGGTGCCAAGGCAGGAGGGGTTCTTGAG9590.10711744606988437No Hit
GTGTGAGGCTTTGTGATGGGGATGGTAGGAACCCGCACCTTTGGCCGGGC9290.10376653534819873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2900.0114.893641
GCGAAAG3650.091.290452
GAATAAG1600.063.3788261
GCGTAGA606.8424524E-660.3607831
TGTATAC6900.058.7894442
CGAAAGC6050.055.076063
GTATACT7150.054.7076573
TATAGAC8900.053.717322
GAGCGAA701.7051518E-551.7378161
GAGCATA450.00962082348.356182145
CAATAGT752.5643558E-548.2886281
GGCTATA1453.8744474E-1044.960892
TAGACCT10750.043.8040054
ATTGCCT1201.978824E-742.2761739
GTATTCC700.001058487241.4133959
TAGGTAC11750.039.50803145
TACTCCT10350.039.206116
ATAGACC12400.039.1393433
ACTCCTA10050.038.9409567
GAACCAT2500.037.686197
Produced by FastQC (version 0.11.5)