FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_E10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_E10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475028
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT15190.31977062404742457No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC10860.2286181025118519No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC10200.21472418467963994No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC7210.1517805266215886No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG6880.14483356770548264No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG6860.14441253989238528No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG6550.13788660878937664No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC6050.1273609134619433No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG5680.11957189891964262No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA5650.11894035719999664No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT5330.1122039121904393No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA5080.10694106452672264No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC5040.10609900890052797No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA4790.1008361612368113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATG5950.054.80015
AGCGAAA1750.053.7975351
CATTAAG6700.049.7210272
CGAAAGC1900.049.5607953
TAACGGC604.933473E-448.35112145
GCATTAA7250.046.947981
TTAAGAT6500.046.8140224
GCGAAAG2050.045.924722
ATTAAGA7150.043.5623133
AGATGTA7950.039.19527
TTGCACG1251.2605062E-534.81281145
GATTAGC3150.034.48561
GTCTAGT5250.033.1061741
ACTGATC2257.930794E-1032.2103048
GTTCGGT900.003646046232.1865581
AAGATGT10800.031.535936
TAGCACA3950.031.181134
TTAGCAC4000.030.784883
TAACGTG1000.006088535328.9831645
TAGTACT4500.028.9801084
Produced by FastQC (version 0.11.5)