FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627435
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAGCATGGCCAGCCACCCATTGAGCGCAAGGTTGTGGAGCTGCCC21680.34553380031397674No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG15720.25054388103947023No Hit
TTCCCAGGCTGCATCAATAATGCTGACCTCTTTGAAGACCAGGTAAACTG11500.1832859180632257No Hit
CAATAATGCTGACCTCTTTGAAGACCAGGTAAACTGGCACCTCAGGAAGG9840.156828994238447No Hit
GTTGTGGAGCTGCCCAGCGTCCAAAAGGTCGAAGTGTATCCAGTAGAGCT9520.15172886434451377No Hit
CCCCCATTGCTGTGCCAGACGCAACTCTACTCGCCCAACCTGCAGGGCCG9430.15029445281184503No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT8720.13897853960968068No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA8380.13355965159737662No Hit
CAACCCACACTGCTCAATTCAGCCGTACGGATTCTGTTGACCTCGTTCTG8350.13308151441982038No Hit
GTTCACGGCCTGTGGCTGGGCACTAGGCCAAGTGCCATCCTTGTTTCGGG7980.1271844892299601No Hit
GTATAGGACATTTTGGTTGTAATCCAGCTATGGGAACGGTCCTGGTAGGA7980.1271844892299601No Hit
GTCTTGAGGGCAGCGTCGAGCACTGTGACTCGTGTGTTACACAATCTCTC7080.11284037390327285No Hit
GACAAGGACTCTGGCACCTTCCCAGGCTGCATCAATAATGCTGACCTCTT6720.10710272777259795No Hit
CCCTTAGGCAGGGGTGTGTTCCTAAAGTCTGGATGTGCACTGGCATGCAT6660.10614645341748548No Hit
CCTCAGGAAGGGTCTAGTGGAAGGTGAGGATTATGTGCTGCTCCCAGCGG6630.10566831623992923No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA6580.10487142094400216No Hit
CCTCCAATGCAGTGTTCACGGCCTGTGGCTGGGCACTAGGCCAAGTGCCA6460.1029588722337772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2800.0111.251471
GCGAAAG3550.087.740652
CGAAAGC3750.083.0743943
GTATAGG2150.060.6498031
TATACAG5650.050.0247275
TTACGCA604.95903E-448.3029144
CTACGGC450.00966451448.2990653
TACAGCT6150.044.782857
ATACAGC6750.040.7988936
GTTACGC750.001488141538.639253
TATGGTC951.03081686E-438.143016
TATAGGA3650.037.706952
CAGTACG1209.573276E-636.2416429
GGGTTAG800.002043937636.221411
TACTGGC1001.3979831E-436.218522
TATACTT3750.034.7864235
TATATAC11900.034.7022673
GTACACC2103.8016879E-1034.4993323
GTAGCGC1301.6618473E-533.4378173
CATATAT4550.033.4351461
Produced by FastQC (version 0.11.5)