FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331342
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5150.049.2278631
CGAAAGC6050.043.1003653
GCGAAAG9000.028.967582
CGATACC1100.00971134826.3420814
GGCGGTT2451.9202398E-623.6630087
GTATATA16650.023.4925251
TATACGT2552.7275055E-622.7265034
GTATACG2953.9112638E-722.0980683
GTATAGG6000.021.7305851
TATAGAC9400.021.5716022
GTATTAG4901.8189894E-1120.6957951
TATATGT10900.020.602414
AATGCGT2205.925374E-419.7625036
TATAAGA7400.019.5726912
ATATATG10450.019.4077683
GTACTAT4202.8881914E-818.9711441
GTATAGA12500.017.963951
GTATTAT9900.017.5600681
CCCGTAC2500.001402439717.3838143
GTATAAG5852.382876E-1017.334941
Produced by FastQC (version 0.11.5)