FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029065
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA22620.2198111878258419No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG22330.21699309567422853No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA19520.18968675448100947No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT18930.1839533945863478No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG18800.18269011189769355No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG17930.17423583544285345No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT17160.1667533149023628No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC17160.1667533149023628No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC16420.15956232113617702No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC15810.15363461005864545No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC14430.14022437844062327No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG14320.13915544693483892No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA14090.13692040833183522No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT12380.12030338219645989No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG12360.12010903101359No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG12130.11787399241058631No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT12050.11709658767910676No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT11840.11505590025897294No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG11610.11282086165596925No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG11290.10971124273005106No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10800.10494963874973884No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT10740.10436658520112918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA6700.051.909051
TAAGATG6450.047.1763155
TTAAGAT6200.046.734694
AGCTATC2250.045.1080979
ATTAAGA6650.043.5721933
GAGCTAT2450.041.4258048
AGGTCGT1953.6379788E-1240.8728336
GTAGAAA8600.040.4309432
CCTTATA9150.040.375712
TTATACT9750.040.1199344
CCCTTAT8850.040.1170961
GGTCGTT2005.456968E-1239.864587
AGCGAAA2005.456968E-1239.8510131
CGAAAGC2005.456968E-1239.8413243
CTTAGAC5250.037.2542273
GTACACC7600.035.2662433
CAATTCG3900.033.4593059
GACAGTA3250.033.4527937
GGATAGC3250.033.4381525
GCGAAAG2603.6379788E-1233.433282
Produced by FastQC (version 0.11.5)