FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_C09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_C09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450408
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA16540.3672226070584892No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG11090.24622120388625426No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT8150.1809470524502229No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT7870.1747304665991723No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC7250.16096516935756025No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG7180.15941102289479758No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG7070.15696879273902772No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT6830.15164029058098435No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT6490.14409157919042292No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC6170.1369869096463651No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC6140.13632084687660967No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG5890.13077032379531447No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA5740.12744000994653737No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC5540.12299959148150122No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG5060.11234258716541447No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC4960.11012237793289639No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG4900.10879025239338555No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA4850.1076801477771265No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG4730.10501589669810482No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC4650.10323972931209037No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT4610.10235164561908314No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC4570.1014635619260759No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG4560.10124154100282409No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG4560.10124154100282409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG2900.057.4947139
AGTAGAA4800.054.3579181
CGAAAGC953.1419404E-853.404273
ATTAAGA3300.048.3181463
GTAGAAA5650.046.175052
AGCGAAA951.9226682E-645.7750851
GCAGGTA2700.045.6287352
TAAGATG3250.044.6063235
ACTGATC2650.043.769568
AGCTATC1505.347829E-1043.4969
CCCTTAT6050.043.126941
CCTTATA6600.039.528642
ACAAGGC4300.038.77558
GACGGGC750.001479950738.680305145
GCGAAAG1157.1493596E-637.81421
TTAAGAT3850.037.6505054
TTATACT6800.037.304454
GAGCTAT1752.4247129E-937.2822848
TAGAAAC7050.037.0096443
TATATTC2750.036.90578
Produced by FastQC (version 0.11.5)