FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409145
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG21740.15427794868519562No Hit
ATCCTATAGCGTCCGTTCTGTCTAGTTGGTGCTTGTCCCCTGCTGGTTGT14780.1048862963002388No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA353.4415105E-582.838959
AGCGAAA6800.070.299751
CGAAAGC6750.069.7474443
AGTACGT855.3517757E-542.6346668
GTAACGT750.00149038738.6307264
AAGCGCG800.002031635536.2691145
ACGCTTG1251.2719916E-534.7738275
TAGAGCG2302.7284841E-1134.647835
CGGTAAT1051.8564926E-434.516239
CAGCTCG11700.032.8346679
GCGAAAG14800.032.2930032
TATAGAC9500.032.014882
TACACGG1601.888895E-631.6948955
GTATTAC6300.031.0414451
GCTATCG950.004734669330.5174438
GTATAAG3850.030.1007943
CGGGTAT1453.478861E-530.01581145
CTACGCT1255.1841047E-428.9720153
TATACAG15550.028.8850125
ATAGACA9950.028.3896663
Produced by FastQC (version 0.11.5)