FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_A12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_A12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244293
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC18340.7507378434912175No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT16020.6557699156340951No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC14430.5906841374906362No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13770.5636673993933514No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG13390.5481123077615814No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG12130.4965348986667649No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10180.41671271792478703No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC8810.36063251914708977No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC8810.36063251914708977No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG8220.33648119266618365No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA8000.327475613300422No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC7510.3074177319857712No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT6830.2795823048552353No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC6670.27303279258922686No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG6650.2722141035559758No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT6430.26320852419021423No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA6420.2627991796735887No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA6370.260752457090461No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA6320.25870573450733336No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT6310.25829638999070786No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT6300.25788704547408237No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC5770.23619178609292937No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT5480.2243207951107891No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA5370.2198180054279083No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG5340.21858997187803172No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG5340.21858997187803172No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC5080.20794701444576802No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG4990.20426291379613826No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT4920.20139750217975957No Hit
TTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAG4750.19443864539712558No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG4750.19443864539712558No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA4740.19402930088050005No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA4720.193210611847249No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA4630.18952651119761926No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC4560.18666109958124055No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG4530.18543306603136397No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT4500.1842050324814874No Hit
TGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGA4440.18174896538173424No Hit
GTTCCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAA4430.18133962086510869No Hit
GTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCACCCGCATAAT4340.17765552021547895No Hit
ATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACTCG4260.17438076408247474No Hit
CCTAAATTCCGTATGGGAGCTTCCTGGTAACTCGTTCTGTTCCCTAATAC4140.1694686298829684No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA3960.1621004285837089No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC3880.1588256724507047No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT3820.1563696053509515No Hit
GTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTAT3760.15391353825119836No Hit
GTAGAAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCC3650.14941074856831754No Hit
GTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAG3620.14818271501844096No Hit
GAATGGAACATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATC3490.1428612363023091No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC3350.1371304130695517No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC3340.1367210685529262No Hit
GATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAA3340.1367210685529262No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC3340.1367210685529262No Hit
GTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAA3280.13426500145317302No Hit
CTTTTGGGCAGCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGG3250.13303696790329644No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT3130.12812483370379013No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA3070.12566876660403695No Hit
CTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGT3060.12525942208741142No Hit
GGGAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTA3010.12321269950428379No Hit
GTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAATGTGCAATA2980.1219846659544072No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA2910.11911925433802852No Hit
TTATCAGCCATTAGCTGGTTTCCTGTGTCCCAGCAAATGCTGAGGTAAGT2850.11666318723827535No Hit
ATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTT2820.11543515368839877No Hit
TGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTGG2810.11502580917177323No Hit
CCATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGT2770.11338843110527114No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA2770.11338843110527114No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG2750.11256974207202008No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2740.11216039755539454No Hit
GTATGGGAGCTTCCTGGTAACTCGTTCTGTTCCCTAATACTCATGCTGTC2720.11134170852214349No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT2720.11134170852214349No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT2680.10970433045564137No Hit
GGTAAGTCTTGATTGCCAATATTGCACATTTCAAACATGAATCCGTAGAC2630.10765760787251374No Hit
GTCTTGATTGCCAATATTGCACATTTCAAACATGAATCCGTAGACCATTT2610.10683891883926269No Hit
ATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAA2580.1056108852893861No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT2570.10520154077276057No Hit
GGGTTTCACCCGCATAATTACACTAGTACACTGTTGGCTTTTTTTTTTTT2550.10438285173950952No Hit
ACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGAGTTAC2520.10315481818963293No Hit
CATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTA2510.10274547367300742No Hit
GTCGAAGAGCAATACTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTA2500.10233612915638189No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC2480.10151744012313084No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA259.3524146E-487.060234145
GCGAAAG750.086.82851
CGAAAGC801.8189894E-1281.435072
GTAGAAA5050.075.9391251
TTAAGAT3500.072.431244
TAAGATG3600.070.433695
TAGAAAC5900.066.252262
ACTGATC1009.094947E-1265.214848
TTAGACG350.0035535162.18588145
AATCCCG350.003573789362.096645
GATATAC350.0035912462.020361
AGAAACA6600.059.2497753
ACAAGGT3950.058.69057
AGATGTA4450.056.980067
AAGGTCG3850.054.5921639
GTGTCCG400.006063241554.3345647
GAAACAA7300.052.5870634
GTACTGA1405.456968E-1251.74726
AAGATGT4900.051.74726
CAGGTAC1152.4956535E-950.376623
Produced by FastQC (version 0.11.5)