FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_A08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_A08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968746
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG57650.5950992313774714No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA52470.5416280428512737No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA52100.5378086722422596No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG48700.5027117531324No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA48010.4955891430777521No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG46260.477524552359442No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG42090.43447921333352607No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG37540.3875112774659199No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC37020.3821435133667649No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG32870.339304626806201No Hit
GATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGG31980.3301174920980319No Hit
CAATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAT30420.31401419980056694No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC30330.31308516370648243No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT29900.3086464357014119No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC29670.3062722323498626No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG29520.30472383885972176No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT29450.30400125523098936No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT28720.29646574024563715No Hit
ATGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA27800.28696892683943986No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA27100.2797430905521158No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC25780.26611722783887626No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT25400.2621946309971861No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC25350.26167849983380576No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT25010.2581688079228198No Hit
GTCTATATGAACTGCTAGGGAAAAATTTCTCGAATACTTCTGGTACATCT23990.24763973218986193No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT22550.23277515468450968No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG22380.23102030872901666No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA22360.23081385626366455No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA21750.22451705607042508No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC21400.22090413792676306No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC21010.21687831485239678No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG20890.21563960006028413No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG20560.21223313438197422No Hit
GTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATACTTCTGGTACA20490.21151055075324185No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG19730.20366535706986144No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG19720.2035621308371854No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA18620.19220724524281907No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG17890.1846717302574669No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC16860.17403942829183294No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG16190.1671232707025371No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA16090.16609100837577653No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA15800.16309744762817086No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT15650.16154905413803No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC15250.1574200048309877No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG15230.15721355236563558No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC15220.15711032613295953No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG15120.15607806380619893No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC14830.15308450305859328No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT14530.14998771607831154No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC14420.14885222751887492No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA14230.14689092909802984No Hit
GCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC14200.14658125040000167No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC13880.14327801095436782No Hit
ATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAA13810.14255542732563542No Hit
GTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCAC13730.14172961746422696No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA13720.1416263912315509No Hit
CCATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCA13720.1416263912315509No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG13540.13976831904338186No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT13370.1380134730878889No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA13250.13677475829577618No Hit
CTGTAAGTCTGGCGACCTTGAGTTAGTTTATCTACCCTTAGGAATGTGTA13210.13636185336507195No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC13080.1350199123402832No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT13070.13491668610760715No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA13060.1348134598749311No Hit
CAATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA13050.13471023364225504No Hit
TGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAAT12990.1340908762461987No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT12990.1340908762461987No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC12990.1340908762461987No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT12930.13347151885014236No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG12730.1314069941966212No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA12550.12954892200845217No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG12490.12892956461239582No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC11910.12294244311718448No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG11840.12221985948845208No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT11510.11881339381014219No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG11470.11840048887943795No Hit
TGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG11430.11798758394873372No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA11400.11767790525070555No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT11300.11664564292394497No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA11160.11520047566648016No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG11010.1136520821763393No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA11000.11354885594366325No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT11000.11354885594366325No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC10990.11344562971098719No Hit
GGATACACCATGGACAGAAAAGGGAAAGTGGACGACAAACACAGAGACTG10980.11334240347831114No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT10970.11323917724563508No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA10960.11313595101295902No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA10910.11261981984957874No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG10790.11138110505746605No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA10550.10890367547324066No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA10380.10714882951774768No Hit
GAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGC10350.10683915081971952No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10290.10621979342366317No Hit
GGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTA10240.10570366226028288No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA10100.10425849500281807No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA10080.10405204253746596No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA10070.1039488163047899No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA10070.1039488163047899No Hit
GTTCATTGATGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTC9960.10281332774535326No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT9890.10209074411662088No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC9850.10167783918591664No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG9830.10147138672056452No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC9820.10136816048788846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC100.0070806826145.06944145
GGTACGC100.0071047917144.904621
CATCGTG100.007105889144.897132
GCCACGC100.007105889144.897132
AGTAGAA6650.0114.398371
TACGGAG203.872968E-4108.700914
CAGGTAC3000.0106.274353
GCAGGTA3000.0106.257892
AGCGAAA1800.0104.653331
GGTACTG3200.0101.917645
CGAAAGC2100.089.712113
GCGAAAG2150.087.612212
CCGCACA502.2610038E-887.041664145
CCGCACG451.2293513E-680.59414145
AAATATA9350.075.187725
CTCATGA300.001938398772.474765
CTATATT300.001941181472.448562
ACTGATC4600.070.906558
GAAAGCA2700.069.7832954
GGTCAAA10050.068.487251
Produced by FastQC (version 0.11.5)