FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R2_A07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R2_A07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758818
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10150.13376066461259487No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG9660.12730325321750408No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT9480.12493114290910337No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC8040.10595426044189779No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC7730.1018689593552077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG3900.055.749679
AGCGAAA1353.6379788E-1253.6388661
AGTAGAA6550.045.326891
CGAAAGC1755.2750693E-1141.3949243
GTAGAAA7500.039.5907022
GGTCCGA1105.3016593E-639.502922
ACAATTC6450.037.075128
TAGAAAC9300.036.6100273
CCTTATA5150.036.5625722
TATACGT800.002042457736.2277266
GTCTAAG3650.035.7102621
ATATAGT3650.035.7102621
TCCGAAC1251.2711684E-534.774034
TATATTC4400.032.938648
TTTAAAC6900.032.546013
GGTCGTT2450.032.535317
ATAGACA4900.032.5245863
CCCGACC2507.094059E-1131.8740943
TATTACA5500.031.6023352
ATATTCA4600.031.5106819
Produced by FastQC (version 0.11.5)