FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_H09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_H09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018046
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT41080.403518112148174No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC38860.3817116318908969No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC38390.37709494462922105No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG24140.2371209159507527No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG22020.21629670957893846No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG21850.21462684397365148No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC20970.20598283378157764No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA19460.1911504981110873No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC18920.18584621912958746No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT18580.1825064879190135No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC17560.17248729428729154No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA17500.17189792995601377No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA16770.16472733059213435No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC16520.162271645878477No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA16430.16138759938156036No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC15870.1558868656229679No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT14640.14380489683177383No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT14260.14007225606701465No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT13970.13722366179917214No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC13230.1299548350467464No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT13000.12769560511018166No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG12380.12160550702031145No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG11980.11767641147845972No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG11540.1133544063824228No Hit
GTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTAT11040.10844303695510811No Hit
GAAACAAGGAGGGGAACCAAAGGTCGAGGTGGCAGAGGGGAAATGAATGG11000.10805012740092293No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA10990.10795190001237666No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC10630.10441571402471009No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA10550.10362989491633974No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG10510.10323698536215456No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA10450.1026476210308768No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA10430.10245116625378421No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT10360.10176357453396015No Hit
GTCTGGGAGAGCCTAAACGCCCGGAGCCCAGCAGGTGCTTTAACGCGAGA10310.1012724375912287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3350.077.8559341
GCGAAAG3900.066.859822
CGAAAGC4050.064.396183
GCATTAA13100.059.176121
GGTGTCG400.006067693254.3369521
TAAGATG14450.052.6525655
GTAGAAA12250.050.2709241
AGATGTA15100.048.473747
TAGACGG604.958612E-448.306645
TCGCACA657.3619816E-444.5841644
CATTAAG17550.044.1604962
TTAAGAT18050.042.5463834
TATAGGG3950.040.3415762
CCGTGAA907.4982396E-540.2634479
GTATAGG4550.039.8072931
AGGGGTC3550.038.7813845
TATAGTG1803.2177923E-936.2299775
AAGATGT21450.035.8094756
TAGAAAC17300.035.1690372
ATTAAGA22300.034.7609443
Produced by FastQC (version 0.11.5)