FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329175
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3900.057.563341
GTACGTA800.002040965536.235119
TATATAC11650.032.3301733
CGAAAGC7650.032.192013
TATACAG7500.030.9066585
GCGAAAG7950.028.2418082
TAACGTA1555.5341985E-528.0403144
CAGCTCG8100.026.8408229
GTATATA10550.026.7707821
TCGCGCC1400.00100203125.8822219
GATCGCG1400.001002251325.8812487
GTTACGC5550.024.796553
GTATTAG4700.024.653071
CAACGTA1801.5248952E-424.156749
ATCGCGC1500.001498110124.156748
ATACAGC10800.024.1540136
ATACTTA7550.023.9940536
TATACTT6050.023.9462745
AACGTAG1851.841845E-423.4932385
GGATCGC1550.001814320523.374856
Produced by FastQC (version 0.11.5)