FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_H01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_H01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196022
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4000.047.0702971
CAATTCG1804.7128524E-628.1852959
TATACAG9250.027.409765
GCGAAAG7000.026.892812
GTATTAG4850.026.875981
CGAAAGC7050.026.7110213
GTATTAT7400.025.4434031
CAGCTCG7700.025.413839
TATATAC16050.024.3682863
TAGACAG9100.023.08535
GAGTATA2353.7433463E-521.5706751
TTATACT7550.021.1074874
TATACTT11450.020.8779265
GTATTAC8500.020.4468251
GTATATA15250.020.4188941
TATACTG8950.020.234665
ATATACT10400.019.5022834
GTAGAAA11300.018.5845971
GGTCGTT1950.00686574218.5782687
TATATAT9750.018.5650582
Produced by FastQC (version 0.11.5)