FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_G06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_G06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355252
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC22640.16705380254004423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3550.034.6846661
CAATTCG3300.030.7436589
GTATTAC16200.029.0613161
CCTTATA6000.028.9665532
TACATCA15900.028.7059975
GCGAAAG4550.028.6535241
GTGATCG2553.0395313E-928.418519
GGCGGTT1804.7195153E-628.1806477
TTACATC16750.028.1112164
TATTACA17050.028.0321452
ATAGACC8100.027.7220733
ATTACAT17850.027.1885223
TATACAG8800.027.1681755
GACAGTA4100.026.511417
ACATCAA17300.026.3858936
TAACGAC6500.025.6308783
TAGTACG3201.2532837E-924.9014034
TAGACCT8750.024.8367234
ACGACTG7300.024.8111215
CGAAAGC5900.023.320532
Produced by FastQC (version 0.11.5)