FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882079
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT9450.10713326130652696No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA9440.10701989277604386No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG9420.10679315571507768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1400.098.3021241
CGAAAGC2550.053.982043
CCTTATA4050.048.2860262
CAATTCG1053.8269773E-641.4232529
TTATACT4900.041.4021074
GTATTAC4850.038.830141
GCGAAAG3750.036.6973762
TATTACA5300.035.5312272
GTCTAAG5550.035.2377471
TACATCA5050.034.4353875
GTACACC1601.8870014E-631.696693
TATAATA2151.8273568E-830.3191342
ACATCAA5850.029.7296496
CTAGTAT1050.00773417727.5998383
CAGCTCG2954.838512E-1027.0304289
TATATAC8050.026.9998423
GACAGTA3005.875336E-1026.5784137
CTAAGGA6600.026.34533
TATTATA2207.548533E-726.337832
GTCTATC2259.183568E-725.7525462
Produced by FastQC (version 0.11.5)