FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890028
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14420.16201737473427802No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11630.1306700463356209No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT10620.11932208874327549No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA9970.11201894771849874No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA9840.11055831951354339No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC8980.10089570215768492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2400.069.402821
CGAAAGC3150.052.8932083
CAATTCG5250.042.8009879
GTACGTA855.365081E-542.614574
AGTAGAA7200.042.2451931
TAGTACC700.001060546541.3970154
GCGAAAG4100.040.6237562
CAGCTCG2950.036.857059
GGTCGTT4250.034.1089257
AGGTCGT4500.033.822786
TAGAAAC10700.031.8197443
TATATAC7100.029.5884533
TCGTTTT4700.029.3026629
CAGGTAC6050.028.7368073
TACTATA1804.741636E-628.161882
GTAGAAA11500.027.7070922
ACGCTCC1050.007715502327.6135399
GGTACTG6700.027.0422785
ACAATTC8850.027.0269838
TATATTC5950.026.7847963
Produced by FastQC (version 0.11.5)