FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436040
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT37260.2594635246929055No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA33920.23620511963455057No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC19060.13272610790785772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4300.050.5375671
TATACCG604.9601716E-448.3049135
GTATTAC19300.038.6581881
TACATCA19450.035.390495
TTACATC20300.033.9027184
ATTACAT20900.032.9282883
TATTACA23900.031.8194332
CGAAAGC7300.030.763143
GCGAAAG7600.029.5426312
CGTAGGG1753.7852278E-628.9910288
ACATCAA24500.028.0996326
TATAGAC21950.027.0570452
GTATAGG6850.026.436931
TATACAG10800.024.8233595
ATAGACA25200.023.2850023
GCGTAAC2202.2446726E-523.0481931
CATCAAA28450.022.9270827
TAGACAT26350.022.544484
TAGGTAC24500.021.609669145
CCTCGCG2404.3527223E-521.1533145
Produced by FastQC (version 0.11.5)