FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_F02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_F02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295735
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATAGGTGACCACTTTGCCCATGGGTTTGAGGAGGTTGTAGCCCTTG27520.21238910734062136No Hit
GTGGAGAACGTGACGGTGTTCGGGACGGCCTCGGCCAGCAAACACGAGGT23120.17843154657395224No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT20990.1619930001119056No Hit
CCTATGGAATGGCCAACCTGCTGACGGGCCCCAAGCGGAACCTGATGGCC19230.14840997580523796No Hit
CTCTAAGACCACTAAAATAACAGAACAGGACTTAAAAAACCCAAGAACCC16020.12363639170046345No Hit
GTCCTACCCTAGCTATGATTCAGTTTCATATTTCATTCTGTTTCTCAGAG15770.12170698483872089No Hit
GACCAGCACAGTGCGGCCCCCGGTGTCTCTCTTCCCGGGGCCCTGAGGGT15580.12024063562379654No Hit
TCCTATGAGAGCACAGAATGGAGAAGCCACGGCTAAGATGCGGTACAAGC13700.10573149602349245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCG100.0070844316145.04565145
AGCGCGT300.001937609672.4836048
GGCGTCT1350.064.429868
TCCTATG4850.056.7452351
CGGCTTT553.2143563E-452.743874145
TTAAGCT5400.048.348553145
TATAGAC8350.047.7068252
CTTGACG803.7402613E-545.3022469
CCCATTA3050.045.1188552
TATATCC3400.044.7468074
ATAGACC9000.044.2715873
GTATTAC3700.043.0634231
GATGATA1651.369699E-939.5365078
TATAGGC800.002044131336.2236064
CTCTTAA11100.035.262299
AATGCCG850.002738053234.128387145
CTATACT2551.8189894E-1234.0928044
TGCACGG850.002755741834.083592
GTATAGC850.002756263434.0822751
CTAAAGC3200.033.9583173