FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_E10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_E10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920876
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT31090.33761331601648864No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC27200.2953709294193789No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC27100.2942850068847489No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG18970.20599950481932422No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG18390.1997011541184698No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC15780.17135857596462498No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG15690.17038124568345792No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA13190.14323318231770618No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA12970.14084415274152004No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC12890.13997541471381597No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC11630.1262927907774771No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT10890.11825696402121458No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC10880.11814837176775159No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT10500.11402186613615731No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT10170.11043832177187807No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG10130.11000395275802605No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG9450.10261967952254157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2700.056.32751
AGATGTA12100.046.7074977
CGAAAGC3300.043.8844033
TAAGATG13450.041.9988375
TTAAGAT14450.040.092534
ATTAAGA14600.039.1803553
GCATTAA15800.038.9607051
CATTAAG16500.038.6140822
GTAGAAA7750.038.313081
GCGAAAG3850.037.6111152
AAGATGT18350.033.550196
TAGTACT6750.033.258244
ACGATCG900.003620387332.23619145
GATTAGC6550.032.0642931
ACTGATC6350.031.95288
TACGGAG1402.7669541E-531.0375985
CATTCGC950.004717087430.539549145
AGCTATC5600.028.4696989
TAGAAAC11050.026.208652
GAGCTAT6100.026.1346978
Produced by FastQC (version 0.11.5)