FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_E09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_E09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277654
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAATGACTGAGCCACCCAGGCGCTCCAAAAGAGTTGAAGAACTCTTAC43381.5623761948324173No Hit
TCTTTATCCAAGGACCAGGAACCACTAGGGCCACCTGTTTGTGAGCGAAA29441.0603124752389665No Hit
GCAGGAAACAGCTCCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGC26530.9555057733726148No Hit
CCACTAGGGCCACCTGTTTGTGAGCGAAACTACCAGAAATACAATGGCAA25170.9065239470708147No Hit
TCCCAGGACTCTGGGATCATGACCTTAGCCAAAGGCAGACACTTAATGAC20010.720681135513985No Hit
GAGCAGGGAGCCTGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGA19050.6861057287127144No Hit
CCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCC16980.6115525077974746No Hit
ATCCCAGGACTCTGGGATCATGACCTTAGCCAAAGGCAGACACTTAATGA14920.537359447369748No Hit
CTCCAAAAGAGTTGAAGAACTCTTACCATATTTCAGGAACTGAAATGGTC13660.49197922594308025No Hit
GACCAGGAACCACTAGGGCCACCTGTTTGTGAGCGAAACTACCAGAAATA13550.4880174605804346No Hit
AGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGC12040.433633226965936No Hit
GGGCAGGGGATGACAAGAGTGCCGCGAAGAGCCAGCAGAGGGAGCCGTCA11950.43039178257831695No Hit
GAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAAC11720.42210809136551247No Hit
AAACAGCTCCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTT11700.421387770390486No Hit
CACTTAATGACTGAGCCACCCAGGCGCTCCAAAAGAGTTGAAGAACTCTT10220.3680840182385271No Hit
GGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGCTCGAT9990.3598003270257227No Hit
GGCCAGAAGCCATCTGGGCAGGGCAGGGGATGACAAGAGTGCCGCGAAGA9970.3590800060506962No Hit
GGAAACAGCTCCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGCAAC9290.33458909289979616No Hit
GTTTGTGAGCGAAACTACCAGAAATACAATGGCAAGGCCAGAAGCCATCT9260.33350861143725646No Hit
ATATAAGCGTGCCCTCTCAGTATTCTGCATAGCAACAAGGCAAGAACCAA9040.32558508071196524No Hit
ACCTTAGCCAAAGGCAGACACTTAATGACTGAGCCACCCAGGCGCTCCAA8900.32054283388677995No Hit
CTCTGGGATCATGACCTTAGCCAAAGGCAGACACTTAATGACTGAGCCAC8690.312979463649002No Hit
GTCTAGACTGGGCTAGAGTGTGTCAGTGGTGGGCGGGCCCTCACAGCGCG8400.30253480951111816No Hit
GTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGG8280.2982128836609593No Hit
CACTAGGGCCACCTGTTTGTGAGCGAAACTACCAGAAATACAATGGCAAG8250.29713240219841963No Hit
AACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACC8220.2960519207358799No Hit
GCTCCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTTCTTTA8140.29317063683577405No Hit
CTCTTACCATATTTCAGGAACTGAAATGGTCACTGGACATATCAGGAAAG8090.29136983439820785No Hit
GCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGCTCGATCCCAGGACTCT8020.2888487109856152No Hit
ATCCAAGGACCAGGAACCACTAGGGCCACCTGTTTGTGAGCGAAACTACC7820.2816455012353505No Hit
GGGCTAGAGTGTGTCAGTGGTGGGCGGGCCCTCACAGCGCGCTGCCAGCC7800.280925180260324No Hit
ACCTGTTTGTGAGCGAAACTACCAGAAATACAATGGCAAGGCCAGAAGCC7690.2769634148976784No Hit
AAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGA7220.2600358719845563No Hit
ATCATGACCTTAGCCAAAGGCAGACACTTAATGACTGAGCCACCCAGGCG7220.2600358719845563No Hit
GCCTGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACCTTAGCCA7200.25931555100952985No Hit
ACCCAGGCGCTCCAAAAGAGTTGAAGAACTCTTACCATATTTCAGGAACT7150.25751474857196366No Hit
CTTCAGGAAAGTCCACCCCTGGGCTCTTTGCTTAGGGAAGAGTTAGCCTG7120.25643426710942396No Hit
GCCAAAGGCAGACACTTAATGACTGAGCCACCCAGGCGCTCCAAAAGAGT7100.25571394613439746No Hit
GTATTAGAGTACTGTGAGGGCAATGACCTGGACTTCTACCTGAAACAGCA7060.2542733041843446No Hit
CTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGCCA7030.2531928227218048No Hit
GTCTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGC6980.2513920202842387No Hit
ATACAATGGCAAGGCCAGAAGCCATCTGGGCAGGGCAGGGGATGACAAGA6940.2499513783341857No Hit
CTCTAATACTGTACAAAACCTAAAGACAAATAAACCGACATTTGACGGTG6920.24923105735915924No Hit
TCTTGGAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAG6690.2409473661463548No Hit
GATCATGACCTTAGCCAAAGGCAGACACTTAATGACTGAGCCACCCAGGC6590.23734576127122245No Hit
ATCATACACTATGACCTCAAACCAGGTATGTCTAACTTTTAGGCAACTGT6450.23230351444603714No Hit
GCGCTGAGCAGGGAGCCTGATAACGGGGCTCGATCCCAGGACTCTGGGAT6440.2319433539585239No Hit
GGCCACCTGTTTGTGAGCGAAACTACCAGAAATACAATGGCAAGGCCAGA6220.22401982323323272No Hit
CCCTGATACTGTGCTTTTAAAAGTTCTCTTATACCTTGTGTTTAGCAAGG5990.21573613202042832No Hit
GGCAAGGCCAGAAGCCATCTGGGCAGGGCAGGGGATGACAAGAGTGCCGC5930.21357516909534888No Hit
TCTGTAATGTGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAG5870.2114142061702695No Hit
CATCTGGGCAGGGCAGGGGATGACAAGAGTGCCGCGAAGAGCCAGCAGAG5870.2114142061702695No Hit
CTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCAC5860.21105404568275624No Hit
GGGCAGGGCAGGGGATGACAAGAGTGCCGCGAAGAGCCAGCAGAGGGAGC5690.2049313173950312No Hit
TCATTATTCTGCTCCCAGGCCACCACCAGTGGGTTGCGAGCGCAGGCATA5660.20385083593249154No Hit
CGCTTACTCTCTGCCCCAGAATCTACAAGTAGTTCGCTCTGAGGCTGGGG5630.2027703544699518No Hit
CTTCTTTATCCAAGGACCAGGAACCACTAGGGCCACCTGTTTGTGAGCGA5610.20205003349492534No Hit
TGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACCTTAGCCAAAG5520.19880858910730623No Hit
GGCTTACTGCAGTAATCCTCTCAGCAGACGTCCATATGACGGATGGCAGG5470.19700778666974003No Hit
GAAATACAATGGCAAGGCCAGAAGCCATCTGGGCAGGGCAGGGGATGACA5460.19664762618222678No Hit
GGCAGACACTTAATGACTGAGCCACCCAGGCGCTCCAAAAGAGTTGAAGA5440.19592730520720034No Hit
GGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGC5390.19412650276963414No Hit
AGCGAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCA5330.19196553984455472No Hit
TCCAAGGACCAGGAACCACTAGGGCCACCTGTTTGTGAGCGAAACTACCA5300.19088505838201503No Hit
CCTGTTTGTGAGCGAAACTACCAGAAATACAATGGCAAGGCCAGAAGCCA5230.1883639349694224No Hit
GAACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAAC5230.1883639349694224No Hit
GGACATATCAGGAAAGAAATCTCAGCACACTGTTCTCTTCAGGAAAGTCC5220.18800377448190914No Hit
GCAGTAATCCTCTCAGCAGACGTCCATATGACGGATGGCAGGAGGAGGAG5150.18548265106931647No Hit
GGGGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGA5090.18332168814423705No Hit
GTACTGGAGACCCGGCCTGAGTCCTGTGGTAAGCCTGCTTGCTGCTTCCG5080.18296152765672385No Hit
TTATAGTACAGTATTGTGCCAGACTGACATACGACATGACATGGAACCAA5030.18116072521915766No Hit
CAGCTCCTGTGACTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTTCTT5020.18080056473164444No Hit
GAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGCTCGATCCCA5010.1804404042441312No Hit
CTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGACC4930.1775591203440253No Hit
GTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTG4890.17611847839397235No Hit
GGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGG4770.1717965525438135No Hit
ACCAGAAATACAATGGCAAGGCCAGAAGCCATCTGGGCAGGGCAGGGGAT4770.1717965525438135No Hit
GATGACAAGAGTGCCGCGAAGAGCCAGCAGAGGGAGCCGTCACGCTTACT4770.1717965525438135No Hit
GGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATA4690.16891526864370765No Hit
GATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACCTTAGCCAAAGG4660.16783478718116793No Hit
GGAACCACTAGGGCCACCTGTTTGTGAGCGAAACTACCAGAAATACAATG4600.1656738242560885No Hit
GAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGCCACCTGTTTGT4560.16423318230603556No Hit
GTTTTATATGTGTTATATAAGCGTGCCCTCTCAGTATTCTGCATAGCAAC4520.16279254035598262No Hit
AAAGCAGGCAGTGAGGTACTGGAGACCCGGCCTGAGTCCTGTGGTAAGCC4450.16027141694338998No Hit
CCAGAAATACAATGGCAAGGCCAGAAGCCATCTGGGCAGGGCAGGGGATG4330.15594949109323114No Hit
CCTGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACCTTAGCCAA4320.1555893306057179No Hit
GAGCCTGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACCTTAGC4310.15522917011820467No Hit
GTGTTATATAAGCGTGCCCTCTCAGTATTCTGCATAGCAACAAGGCAAGA4250.15306820719312525No Hit
GTACAGTATTGTGCCAGACTGACATACGACATGACATGGAACCAATTCGT4240.15270804670561203No Hit
ACTCTGGGATCATGACCTTAGCCAAAGGCAGACACTTAATGACTGAGCCA4230.15234788621809878No Hit
TGACAAGAGTGCCGCGAAGAGCCAGCAGAGGGAGCCGTCACGCTTACTCT4230.15234788621809878No Hit
GCAGGGGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCC4170.15018692329301936No Hit
GTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTGGCATTCCCTTCCCCC4120.1483861208554532No Hit
GTCCTGTGGTAAGCCTGCTTGCTGCTTCCGTGTGTGTTTGAGTGAGAGTG4090.1473056393929135No Hit
AAGCAGGGGTTCTTGGAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAG4070.14658531841788702No Hit
GTTCTCTTCAGGAAAGTCCACCCCTGGGCTCTTTGCTTAGGGAAGAGTTA4040.1455048369553473No Hit
AAAGCAGGGAGTGCGGCGTGAGGTAATGCCTTGATCATTATTCTGCTCCC4040.1455048369553473No Hit
GAAATGGTCACTGGACATATCAGGAAAGAAATCTCAGCACACTGTTCTCT3990.1437040345177811No Hit
CCACAGGACTCAGGCCGGGTCTCCAGTACCTCACTGCCTGCTTTCCTCTG3960.1426235530552414No Hit
ATAATGGATCGCGCCTCTTTCTCCGACATTAATTTGTGCTGTTTCAGGTA3910.14082275061767524No Hit
GTGGTAAGCCTGCTTGCTGCTTCCGTGTGTGTTTGAGTGAGAGTGCGTGC3890.14010242964264877No Hit
CCAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGCCACC3830.13794146671756935No Hit
ATCCATTATTATGCAGATTGTGAATGCTTTAAAGTACTTAAATGAAATAA3720.13397970135492374No Hit
CAGGGGGAGTGGGAGAGGGAGAAGCAGGCCTTGCGCTGAGCAGGGAGCCT3710.13361954086741054No Hit
GACTCGAGGAACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGA3710.13361954086741054No Hit
GAGTAAGGGTGTGCATCTATTTGTGATAACCTGTTAGTTTTTCTTTCAAT3680.13253905940487082No Hit
AGCGAAAGCAGGAAACAGCTCCTGTGACTCGAGGAACTTGTGGTCTCCAA3640.13109841745481787No Hit
AGGAACTTGTGGTCTCCAACAAGAGCAACTTCTTTATCCAAGGACCAGGA3630.13073825696730462No Hit
GGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGCTCGATCCCAGGACTC3540.1274968125796855No Hit
AAAGCAGGCTTACCACAGGACTCAGGCCGGGTCTCCAGTACCTCACTGCC3530.1271366520921723No Hit
GGCTTACCACAGGACTCAGGCCGGGTCTCCAGTACCTCACTGCCTGCTTT3500.12605617062963256No Hit
CCAAAAGAGTTGAAGAACTCTTACCATATTTCAGGAACTGAAATGGTCAC3420.12317488672952669No Hit
CTCCAGTACCTCACTGCCTGCTTTCCTCTGCAGCTGAACATCTGGCCACA3420.12317488672952669No Hit
ACATTGTACACCCATATCACAATCACACACAAACCCAGGCCCACATGGAA3410.12281472624201344No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA3410.12281472624201344No Hit
TATCAGGAAAGAAATCTCAGCACACTGTTCTCTTCAGGAAAGTCCACCCC3380.12173424477947374No Hit
GCAGGGAGCCTGATAACGGGGCTCGATCCCAGGACTCTGGGATCATGACC3360.12101392380444727No Hit
AAAGCAGGCTTACTGCAGTAATCCTCTCAGCAGACGTCCATATGACGGAT3360.12101392380444727No Hit
CAAGAGTGCCGCGAAGAGCCAGCAGAGGGAGCCGTCACGCTTACTCTCTG3340.12029360282942078No Hit
GAGTTGAAGAACTCTTACCATATTTCAGGAACTGAAATGGTCACTGGACA3330.11993344234190755No Hit
GAAGAACTCTTACCATATTTCAGGAACTGAAATGGTCACTGGACATATCA3320.11957328185439432No Hit
GTGTAAGTGTTGCAGCCATGGCAACGGCTGCCGCATTTGGCATGTTGCTG3290.11849280039185461No Hit
TCTCAGCACACTGTTCTCTTCAGGAAAGTCCACCCCTGGGCTCTTTGCTT3290.11849280039185461No Hit
GCCAGAAGCCATCTGGGCAGGGCAGGGGATGACAAGAGTGCCGCGAAGAG3280.11813263990434136No Hit
GAGATAGAGAGAGAGAGATGGGAGAAAGAGAAGGAGAGAGAGATGGGAGA3270.11777247941682815No Hit
CCATATGACGGATGGCAGGAGGAGGAGGCGCAAGGGCAGTCTAGACTGGG3220.11597167697926195No Hit
GGATATGGGTGCCAGGAGAGGAAGGAAGAGAGAGAATGAGAAGGGGAGGA3140.11309039307915607No Hit
CTCTTATACCTTGTGTTTAGCAAGGAGACGTTATAGTACAGTATTGTGCC3130.11273023259164283No Hit
TCACTGGACATATCAGGAAAGAAATCTCAGCACACTGTTCTCTTCAGGAA3110.11200991161661636No Hit
GGGGATGACAAGAGTGCCGCGAAGAGCCAGCAGAGGGAGCCGTCACGCTT3090.1112895906415899No Hit
AGCCAAAGGCAGACACTTAATGACTGAGCCACCCAGGCGCTCCAAAAGAG3060.11020910917905018No Hit
GTGTATGATGGGAGGTTTTATTTCATTTAAGTACTTTAAAGCATTCACAA3050.10984894869153695No Hit
CAACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGCCACCT3030.10912862771651048No Hit
GTTAACAACTGTGCCCACCAGGCAGTAAATGGGAACCATCTTCCGAACAC3020.10876846722899725No Hit
GAGCCACCCAGGCGCTCCAAAAGAGTTGAAGAACTCTTACCATATTTCAG3020.10876846722899725No Hit
ACTCTTACCATATTTCAGGAACTGAAATGGTCACTGGACATATCAGGAAA3000.10804814625397076No Hit
AAGCAGGCCTTGCGCTGAGCAGGGAGCCTGATAACGGGGCTCGATCCCAG3000.10804814625397076No Hit
TTACAGCACCAGTGTGTTAACCAACGGCCATACCCATGCAAGTTTATTGT2970.10696766479143106No Hit
GTATTGTGCCAGACTGACATACGACATGACATGGAACCAATTCGTGGGAC2920.10516686235386488No Hit
AAACTACCAGAAATACAATGGCAAGGCCAGAAGCCATCTGGGCAGGGCAG2900.10444654137883841No Hit
GAACCACTAGGGCCACCTGTTTGTGAGCGAAACTACCAGAAATACAATGG2890.10408638089132516No Hit
CATCAGAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTT2880.10372622040381195No Hit
AACAAGAGCAACTTCTTTATCCAAGGACCAGGAACCACTAGGGCCACCTG2870.10336605991629871No Hit
GTCCACCCCTGGGCTCTTTGCTTAGGGAAGAGTTAGCCTGGTATCCCCAG2810.1012050969912193No Hit
CGTATACATGTTACAGCACCAGTGTGTTAACCAACGGCCATACCCATGCA2810.1012050969912193No Hit
GGGTGGATCTGTCTGATCACAAGTGCACGTATACATGTTACAGCACCAGT2790.10048477601619281No Hit
GCTACATAGTGTGAGTAATGGGAAAAACAAAGCTGAGCTTGCCAGAGCAG2790.10048477601619281No Hit
GTGAGGTACTGGAGACCCGGCCTGAGTCCTGTGGTAAGCCTGCTTGCTGC2790.10048477601619281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACG100.007076782145.07762145
GGCGTCT203.8714978E-4108.6905068
GCAGGAA4500.094.9350741
CGAAAGC4400.092.1391753
GTTGGAT406.158625E-790.4938353
CAGGCTT1500.077.249235
GCGTCTC300.001938466272.4603359
GTTAACA702.8303475E-972.408111
CTTACTG1300.072.3950653
GCGAAAG5600.072.3689962
AGGAAAC5650.070.473073
GAAAGCA6100.066.4854
TGTTGCA902.8194336E-1064.409188
TGTAAGT902.8558134E-1064.327992
AGGCTTA1750.062.0864686
GGGTTAA350.003581739362.0640951
GGTTAAC350.00358941762.0305672
GTGTTGC954.5656634E-1061.0082247
AACTGTG851.3082172E-859.6624577
GGCTTAC2550.059.6302071
Produced by FastQC (version 0.11.5)