FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_E08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_E08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013017
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG134451.327223531293157No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC105881.0451947005825173No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA99860.985768254629488No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA93990.9278225340739592No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG89120.8797483161684355No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC81050.800085289782896No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG78870.7785654140058854No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG72730.7179543877348554No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG69660.6876488745993404No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA68120.6724467605183329No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT63920.6309864493883124No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT58490.5773841899987858No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC55070.5436236509357691No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC47600.4698835261402326No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG47270.46662593026573096No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT45400.4481662203102218No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT43280.42723863469221146No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC39220.3871603339331916No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC39180.38676547382719145No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA37300.3682070488451823No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC34090.3365195253386666No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT30820.3042397116731506No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30550.3015744059576493No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC29010.28637229187664176No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC28380.2801532452071387No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA27130.26781386689463255No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG26810.264654986046631No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG26760.26416141091413076No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA26270.25932437461562835No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG26270.25932437461562835No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT25990.256560353873627No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC25740.2540924782111258No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC25610.2528091828666252No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA25400.2507361673101241No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG25320.24994644709812375No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC25120.24797214656812275No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT24810.24491198074662124No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA24100.23790321386511776No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT23840.23533662317611648No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC23310.2301047267716139No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT23260.22961115163911364No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG22710.22418182518161098No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT22590.22299724486361036No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA22230.21944350390960865No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA21720.21440903755810614No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA21570.21292831216060537No Hit
CATTTGGAAGTTTGATTTCCATTTCATCGACGAACGGGGTGAATCAATAA20910.20641312041160217No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC20700.20434010485510115No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA20620.20355038464310074No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA20420.2015760841130998No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA19980.19723262294709765No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG19590.19338273691359573No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC19570.19318530686059562No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG19450.19200072654259503No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC18620.183807379343091No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATAT18580.1834125192370908No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA18230.1799574933095891No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA18150.1791677730975887No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT18010.177785762726588No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG17910.1767986124615875No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG17880.17650246738208736No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG17870.1764037523555873No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG17810.17581146219658703No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA17790.17561403214358692No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG17640.1741333067460862No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA17560.1733435865340858No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG17460.1723564362690853No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA17280.17057956579208444No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC16970.1675193999705829No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG16530.16317593880458078No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA16280.16070806314207955No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA15810.15606845689657725No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG15740.15537745171107692No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG15080.1488622599620737No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT15040.1484673998560735No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC14890.14698667445857275No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA14760.14570337911407213No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA14690.14501237392857177No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT14680.14491365890207172No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT14460.14274192831907068No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA14450.1426432132925706No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA14440.14254449826607055No Hit
GTATATGTAACACAACAGAGAGTCACCCAGGGGAGTGGAGGAAGGCTCTA14200.1401753376300694No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT13970.13790489202056827No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG13890.13711517180856786No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT13840.13662159667606763No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC13720.13543701635806704No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG13640.13464729614606666No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA13500.13326528577506597No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG13470.1329691406955658No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA13220.1305012650330646No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA13210.13040255000656453No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA13100.12931668471506402No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG12790.12625651889356249No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT12640.12477579349606176No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG12600.12438093339006157No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA12560.12398607328406137No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCTATTCCACCATGTTACGGCC12560.12398607328406137No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA12500.12339378312506107No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG12470.12309763804556093No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA12460.12299892301906089No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA12320.12161691264806021No Hit
CTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA11980.11826060174705855No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA11980.11826060174705855No Hit
ACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAG11840.11687859137605786No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT11830.1167798763495578No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC11730.11579272608455732No Hit
CTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATG11630.11480557581955682No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC11580.11431200068705658No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC11580.11431200068705658No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT11490.11342356544855614No Hit
GTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACGGGGTGAATC11400.11253513021005572No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA11310.11164669497155527No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA11230.11085697475955489No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA11200.11056082968005473No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG11160.11016596957405453No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA11130.10986982449455439No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG10990.1084878141235537No Hit
ACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAG10760.10621736851405257No Hit
GTTCATTGATGCTTAATGCTGGGCCTATTCCACCATGTTACGGCCAGAGG10720.1058225084080524No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT10480.1034533477720512No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA10430.10295977263955096No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA10380.10246619750705073No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA10260.10128161718905013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA202.1366523E-6144.917661
CGGAACC151.2330385E-4144.91054
CCGATCC202.137178E-6144.91054
TACCGAT202.1398064E-6144.874732
AGCGAAA2600.0125.409521
GGGTATA256.4466567E-6116.065994145
ACCGATC256.486378E-6115.922683
GCAGGTA15200.0114.851352
AGTAGAA14450.0113.3266141
CAGGTAC15800.0110.970283
GTGTAAG203.8748162E-4108.688251
TCGGACA404.836693E-9108.682884
GGTACTG16950.0103.024065
ACTGATC18800.091.747928
GACGCAA259.3655963E-487.04949145
CGCGGAA259.407986E-486.95065
TACGGAG259.4098324E-486.94634
ACCGAGA259.411679E-486.942013
GCGAAAG3750.086.9248352
GTACTGA20250.085.877146
Produced by FastQC (version 0.11.5)