FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185584
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG49290.4157444769834951No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT29920.25236507915086576No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA28370.23929135345956087No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA25060.21137262311232272No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG17700.14929351273296534No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT17300.14591964803843507No Hit
GTATGAAAAGATCTCCAGAAGGAGTGCTCGATCTTGCCCACGCTCTTGAT16730.1411118908487294No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT16180.13647282689375023No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC14550.12272432826353932No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA14120.11909742371691925No Hit
GTGCAGATACTGGGTGTTTTTTCCCCTCGGGACCTGTTTCACGATGCATC13860.11690441166547456No Hit
GTATAGCCCAGTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGC13790.11631398534393175No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA13370.11277142741467497No Hit
TCCCTGGACAGGCCGGCTGGGACATCACAGCAGGTGCTACAGCCTCCCGA12990.10956625595487118No Hit
GGCCTGTGCTGCTCGACACGCGGAGGAACAGCAGCAACAGGACCCCGACA12240.10324025965262688No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT12170.1026498333310841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5300.072.421321
GATCGCC702.3272332E-762.172474145
TAGTCGT400.006063023654.3480876
ATAGTCG400.00606504954.3434945
GCGAAAG7800.049.2031252
TTCGACA450.00967133948.29314
GTACGGC450.00967295248.2910583
CGAAAGC8150.047.1060073
TAGACCT5150.045.0110444
TTATACC1851.8189894E-1243.0722244
GTCGCAT1201.9907748E-742.245761
CCTTCGT700.001058197141.416818
TATACAG19950.041.0413675
TTACATC5750.037.79464
TACAGCT20700.037.113497
TATATGT10550.037.0781144
AGTATAC1001.3939303E-436.2397088
AACCGGT1604.6109562E-836.232066
CTATACC1604.6264176E-836.2182963
CAGCTCG21600.035.9041529
Produced by FastQC (version 0.11.5)