FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA22.4096385542168677No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC22.4096385542168677No Hit
CCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTGGG11.2048192771084338No Hit
GACTATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTG11.2048192771084338No Hit
ATTTGCAGGCCTACCAGAGGCGAATGGGAGTGCAGATGCAGCGATTCAAG11.2048192771084338No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA11.2048192771084338No Hit
ATACTGTTGGGATGGGCTACAATCATCCGACGATTTTTCTATCATAGTGA11.2048192771084338No Hit
GCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTAGTGTTGTCCCC11.2048192771084338No Hit
AGATGAAATGGGGCATGGAAATGAGGCGCTGCCTTCTTCAGTCTCTTCAG11.2048192771084338No Hit
TATATAAACAATTCGACAATAATTGATGGCCATCCGAATTCTTTTGGTCC11.2048192771084338No Hit
AGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGAACGTTATTTCTC11.2048192771084338No Hit
GAGTAGGATGCAATTTTCCTCATTGACTGTGAATCTGAGAGGATCAGGGT11.2048192771084338No Hit
GCTGACATGAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAA11.2048192771084338No Hit
CATTATACATAATACAAAGTAGTCACCCGACGTAGCCCTAGTAACGGAAT11.2048192771084338No Hit
GATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTAC11.2048192771084338No Hit
GGACAAGAGACTGAAATGGTTCAAATTCCATTTTGTTGTATAACATTGTG11.2048192771084338No Hit
AGTAGAAACAAGGTCGTTGTAAACCAATTAGACAATAATTGATGTTCATC11.2048192771084338No Hit
TTTTGGTTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGAAATCTG11.2048192771084338No Hit
AAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAAT11.2048192771084338No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTT11.2048192771084338No Hit
GATAAAACCACACTTTAAAAGAAAACGGAGAGGAAGAGACCACATGACCA11.2048192771084338No Hit
ACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAA11.2048192771084338No Hit
GAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTGATGGTGGCGTACA11.2048192771084338No Hit
GGGGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTC11.2048192771084338No Hit
GAACAGATGTACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAG11.2048192771084338No Hit
GCATCAATGAAACCTGGTAACATGATTGGAATGTTAACAATGATAAATAC11.2048192771084338No Hit
ATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATCATAAGTCTGG11.2048192771084338No Hit
GGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGTTG11.2048192771084338No Hit
ACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCAA11.2048192771084338No Hit
CTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTA11.2048192771084338No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT11.2048192771084338No Hit
CGAAGCGCCCTGTCCTCCATATGCCCATTGTGAAAAATTACTCAAAAGGA11.2048192771084338No Hit
CCCATACACACAAGCAGGCAGGCAGGATTTATGTGCAACTGATCCCCTCA11.2048192771084338No Hit
ATAAAGAACAACATGAAAAAAAATGACCTTGGACCTGAAACGGACCAGAT11.2048192771084338No Hit
GACTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCAT11.2048192771084338No Hit
CAATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGTCATTGTTTATCA11.2048192771084338No Hit
GCAAGAAGACATTCTTTGGCTGGAAAGAGCCTAAAATAGTCAAACCACAT11.2048192771084338No Hit
CGTTTATCAGACAAACAACAATCTAAAGAAGAGGCATTGTCTGTTGAAAT11.2048192771084338No Hit
TCCATTAGCTCCCATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTAT11.2048192771084338No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCAC11.2048192771084338No Hit
AGCAAATGTAGGACCAAAGAAGGAAGACGGAAAACAAACCTGTATGGGTT11.2048192771084338No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA11.2048192771084338No Hit
CCATACAGCGTTGGAACAGGCAAAGGATACACCATGGCCACAGTACGCAG11.2048192771084338No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT11.2048192771084338No Hit
GAAGACCTCATTTTCCTGGCACGGTCAGCACTCATTCTGAGGGGATCAGT11.2048192771084338No Hit
CCATGGTATTGAAGTAAAACGGCAGTGTTTACAGACTCAATCTAGTTATG11.2048192771084338No Hit
CCGACCAAATGAATTCGGATGAAAATCAAAAAGATTAAAAAAAATTAAAA11.2048192771084338No Hit
ATTCGACACTAATTGGTGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT11.2048192771084338No Hit
GGAAGACGGAAAACAAACCTGTATGGGTTCATTATAAAAGGAAGGTCTCA11.2048192771084338No Hit
GAAAATGACCGGGGAAGGACACCCCGGCCGCATCCAGACCCACGCCCTGG11.2048192771084338No Hit
GTTTAAGCACCCTGCGCCTCGATCTGCTCCCGGGTCAGGCCGAAGCGCCG11.2048192771084338No Hit
CTTTATCACTGTTACTCCAATAATCATGTAAGAGGAATCATTTACTCCAG11.2048192771084338No Hit
GTTGGAGAGCATGCTTGAGGGCGAGGCTTATTTCAAATAGAAAGAAATGA11.2048192771084338No Hit
GGAATGTGTAGTTGCAACTGCATCATATTCCATGCTTTTGGCTGGACCAT11.2048192771084338No Hit
GGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTGTCGGAAGAAT11.2048192771084338No Hit
ATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAG11.2048192771084338No Hit
GCTCTACATGTATAAAATCTTCTCTACAGCAATTCCCCTATCTTGATAAA11.2048192771084338No Hit
AGAGACGAATTTCCATTATTAAAGATAGGCATGACCACGGACACAACCTA11.2048192771084338No Hit
CCAGAAATCAACCCGAGTGGATCAGAAACATCCTGAGCATGGCACCCATA11.2048192771084338No Hit
TATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAG11.2048192771084338No Hit
TCGTATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTT11.2048192771084338No Hit
TCACTGACTTCTTCTTGAGAAAATATTAAGTTACATCTTTGATCTCTAAA11.2048192771084338No Hit
GTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATCTCTCTTGGAAAT11.2048192771084338No Hit
AACAATAAAAAATGAACCATAATAACGCAACATGTTCAGAAAATAGAACA11.2048192771084338No Hit
GTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCT11.2048192771084338No Hit
TCTTTTCTCCTTTTGGAATTTCAGTTACATGTTTATTAGACCATTTGATA11.2048192771084338No Hit
TATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGCCGCTT11.2048192771084338No Hit
AGACAATAAATGGCCAGTAGGAGTCTATGGGATTCCTTACGTCAGTCCGA11.2048192771084338No Hit
CCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAACA11.2048192771084338No Hit
TGCTCTCAATCTGCTGAAGAGACTGAAGAATGCAGCGCATCATTTCCATG11.2048192771084338No Hit
GAGCAGTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGA11.2048192771084338No Hit
AATGAAGCAGCTGAGGAAAGAACAGTGCCAACAATAACACCTTGTGGGAG11.2048192771084338No Hit
GAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGC11.2048192771084338No Hit
CCCATCACATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAAGCACTTT11.2048192771084338No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG11.2048192771084338No Hit
NGAGACACCAGTTCTGTGGGCCCCAAGGAGGCACGACTGTCGGGCCCTCA11.2048192771084338No Hit
AACAAAGACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAAT11.2048192771084338No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA11.2048192771084338No Hit
GCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGG11.2048192771084338No Hit
CAGGATATACAGGGGCGTTGGGCGCGCAACGGCCGAGGAGTGTGATCTAA11.2048192771084338No Hit
ATCTGATACAAATATACATTCATTTGATTGCCATTGAACCCAGAGCAACT11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCCA50.0145.09
AGAAGAG50.0145.04
AAGAGCT50.0145.06
GAGCTCC50.0145.08
GAAGAGC50.0145.05
AGAGCTC50.0145.07
GACGTGC50.0145.0145
AAGAAGA50.0145.03
AAAGAAG50.0145.02
GAAAGAA50.0145.01
Produced by FastQC (version 0.11.5)