FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_D08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_D08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942698
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC147701.5667796049211942No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG109721.1638934207986014No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG108611.1521187060967564No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG105331.117324954545358No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA102431.0865621864054023No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG98071.0403119556846414No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA90880.9640415063997166No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG67440.7153934770202123No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA66620.7066950391323626No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC64610.6853732584560485No Hit
CATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT58230.6176951685481459No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT54070.5735665080439335No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG53240.5647619916452565No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT45040.4777776127667609No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC43320.4595321089044423No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC41150.4365130720548892No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG38560.40903873775058397No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT36490.3870804860093052No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT35900.3808218538704866No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT35880.3806096968488317No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT34900.37021400278774325No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC32250.34210319741847334No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG31600.33520809421469017No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG30770.3264035778160132No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30360.32205435887208844No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC30050.31876592503643797No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA28900.30656689629128314No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG28560.30296022692315033No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG28280.29999002861998225No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACATAG28230.2994596360658451No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA28140.2985049294683982No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA27740.2942617890353008No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA27290.2894882560480663No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA27020.2866241362557256No Hit
CACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAA26960.28598766519076096No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC26860.2849268800824866No Hit
CAAGCCAAATTCATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAG26550.2816384462468362No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG25210.2674239257959601No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG25200.2673178472851327No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG24150.25617960364825215No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG23890.2534215623667389No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT23710.25151214917184506No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG23070.2447231244788893No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT22700.24079821957827427No Hit
TTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATG22290.2364490006343495No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA22170.23517605850442028No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG22050.2339031163744911No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG21870.23199370317959728No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA21780.23103899658215038No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC21400.2270080131707079No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA21380.22679585614905304No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG21120.2240378148675398No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC21050.22329526529174773No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA20960.22234055869430083No Hit
GTTTTCAAAGAATTCCTTGGTCATGTCTTTTTGACAGAAGACTCGGCCTC20930.2220223231618185No Hit
CATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT20860.2212797735860265No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC20700.21958251741278756No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT20410.21650624059879198No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG20110.21332388527396895No Hit
GATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCTGGACAGTGGTG20100.21321780676314153No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATAT19320.2049436829186017No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG19240.20409505483198223No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA18970.20123093503964154No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA18830.19974583588805747No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA18540.1966695590740619No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT18480.1960330880090973No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC18190.19295681119510172No Hit
CCTATATGAACTGCTAGGGAATTGCAGCACTTCTGGTACATCTGTTCATC18190.19295681119510172No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG18100.19200210459765482No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA18070.1916838690651725No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA17900.18988053438110614No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT17600.18669817905628314No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC17470.1853191584155265No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT17280.18330366670980527No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC17230.1827732741556681No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC17070.18107601798242914No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT17050.1808638609607743No Hit
GAATTCCTTGGTCATGTCTTTTTGACAGAAGACTCGGCCTCAATCATGCT17010.18043954691746455No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG17000.1803334684066371No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC16900.1792726832983628No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA16760.1777875841467787No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA16530.17534777839774773No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA16490.174923464354438No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA16400.1739687577569911No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT16180.17163503051878756No Hit
GTCAAAAAGACATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGG15920.1688769892372743No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC15810.16771012561817253No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC15790.16749796859651767No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA15770.16728581157486277No Hit
ACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAG15710.1666493405098982No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT15710.1666493405098982No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT15350.16283051412011057No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA15300.1623001215659734No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG15260.16187580752266367No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG15150.16070894390356188No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG15080.15996639432776988No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA15020.15932992326280526No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA14780.15678403900294685No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA14630.15519286134053537No Hit
CATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTTT14540.15423815474308844No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG14400.15275305559150437No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG14300.15169227048323006No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC14170.1503132498424734No Hit
CTTTCTCTTTGACAGAAGACTCGGCCTCAATCATGCTCTCAATCTGCTGA13930.147767365582615No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA13790.14628226643103093No Hit
CTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATG13770.14607010940937606No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG13190.13991755578138493No Hit
CTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA13190.13991755578138493No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC13160.13959932024890262No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGC12950.13737167152152652No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT12630.13397715917504863No Hit
CAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAG12550.13312853108842915No Hit
TTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCA12530.1329163740667743No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG12460.13217382449098228No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA12040.12771852703623007No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA11860.12580911384133625No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT11860.12580911384133625No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA11720.12432401468975218No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA11650.12358146511396015No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC11540.12241460149485837No Hit
GCACACATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCA11350.12039910978913713No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG11340.1202930312783097No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG11200.11880793212672563No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA11120.11795930404010617No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT11070.117428911485969No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT11070.117428911485969No Hit
ACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAG11030.11700459744265926No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA10990.11658028339934952No Hit
GAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAA10800.11456479169362828No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCAT10670.11318577105287166No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC10660.11307969254204422No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC10660.11307969254204422No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA10470.11106420083632298No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT10390.1102155727497035No Hit
GTAAGGTCCTGCACACATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTC10360.10989733721722121No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC10350.10979125870639378No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC10320.10947302317391149No Hit
TCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGA10240.108624395087292No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG10230.10851831657646457No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA10210.10830615955480971No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT10160.10777576700067254No Hit
GCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGA10130.10745753146819025No Hit
CCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGG10040.10650282487074333No Hit
TTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCT10030.10639674635991589No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC9940.105442039762469No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG9930.10533596125164156No Hit
GGATACACCATGGACACAGTAAACAGAATGATGCCGTTGCAACTACACAT9900.10501772571915927No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACA9860.10459341167584954No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT9790.1038508621000575No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC9780.10374478358923006No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAC9770.10363870507840263No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA9690.10279007699178315No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCA9610.10194144890516368No Hit
ATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCT9510.10088066379688936No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC9490.10066850677523449No Hit
GGGTGAATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGC9490.10066850677523449No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT9430.10003203571026989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCACG100.007101442144.927267
GCAGGTA22300.0120.167352
CAGGTAC22950.0116.77633
AGCGAAA2800.0113.817041
GGTACTG24100.0110.926995
TTGTACG203.881079E-4108.643541
AGTAGAA13450.0107.701151
ACTGATC26950.099.217378
AGCAGGT27050.098.803361
GTACTGA27200.098.018226
CTATATT1050.096.736305145
TACTGAT28950.092.613287
GAGCGAA554.2018655E-1092.1823961
CTCATGA406.143255E-790.560315
AGGTACT31150.087.207734
CCGGAGA259.4172126E-486.9286654
ACCGGAG259.4211847E-486.919443
ATACCGG259.4231707E-486.9148251
TGTACGA259.4251573E-486.910222
GGTCAAA10300.085.086531
Produced by FastQC (version 0.11.5)