FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236452
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA51810.41902152287351224No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG37250.3012652331024577No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT27480.22224882162833656No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT24480.19798584983485001No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT23900.19329500862144264No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT21360.17275235916962406No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG21140.17097307457143504No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA20190.1632898001701643No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG19860.1606208732728808No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA19850.16053999670023583No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG17990.14549695418827419No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC17110.13837981579551814No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC16790.13579176547087957No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC16480.133284591718886No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC15610.12624832989877488No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA15580.12600570018084No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT15320.12390290929207119No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15060.12180011840330235No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG14860.12018258695040325No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC14760.11937382122395371No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT14550.11767541319840966No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA14410.11654314118138026No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG14340.11597700517286559No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA14020.11338895484822702No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC13590.10991126222449396No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC13430.10861723706217466No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG13280.10740408847250034No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG12930.10457340842992692No Hit
CTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAA12910.10441165528463701No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA12840.10384551927612232No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG12710.10279412383173792No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT12680.10255149411380306No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG12400.1002869500797443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA6050.088.590811
GTAGAAA9000.058.7408182
AGCGAAA1450.054.9461251
CAATTCG4700.052.4230049
CCTTATA17900.048.954432
TTATACT17950.048.4283264
CCCTTAT18350.047.3651
GCGAAAG1900.045.7446171
GACAGTA3900.040.8755657
CAGGTAC4000.039.8407753
TATATTC4450.039.081798
CCTATAC750.001489794838.633483
GGTACTG4300.037.0686845
CGAAAGC2401.8189894E-1236.2100942
ATATTCA5000.034.7842039
TCTGCCG1051.8629036E-434.4955754
ACAAGGC10750.034.3783458
ACTGATC4650.034.2841158
GATTGCG850.00273473534.136707145
TAAGATG1707.870585E-834.095265
Produced by FastQC (version 0.11.5)