FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533765
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG20690.134896806225204No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA16110.10503564757312887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4050.051.8685341
CGCCAAT3100.030.383784
AACTACG1204.0680618E-430.1968947
CGAAAGC7750.028.0429083
GCGAAAG8700.025.8092352
TATACAG17250.024.3636055
TATAGAC13750.023.705142
TATACTG7850.023.0766645
ATAGACC8250.022.8308943
TATATGT16550.022.7648874
TATATAC30300.022.4744343
GGTTCGC1952.6321938E-422.2868822
CAGCTCG14150.022.0263359
GGCGGTT3706.686605E-921.5458937
GTATATA27450.021.1109921
CGGTTCG2104.3409297E-420.6963141
GTATTAG5700.020.3332181
GTATTAC13900.020.3240761
GTATAGG5353.6379788E-1220.3094641
ATATACT17300.020.1027434
Produced by FastQC (version 0.11.5)