FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_C12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_C12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132102
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAAGACACACGCCACTACCAGAGACAACAACAGATACACCTCAACAT16961.2838564139831343No Hit
ATATTCATAGTTGGAGCCATTTTTTATGTCCTCCTAACTCTGCAGACCAC15941.2066433513497146No Hit
ATTCATAACGGGTTAAGACACACGCCACTACCAGAGACAACAACAGATAC12220.9250427699807724No Hit
GGATGAACTGGCATGTGTCAGTTGGAAGATCAGCTGGAGAGAACTTTGGG11880.8993050824362991No Hit
TCCCAGACCCACCCCCCTCCGTATTCATAACGGGTTAAGACACACGCCAC10370.7849994701064329No Hit
GCATTATATTCATAGTTGGAGCCATTTTTTATGTCCTCCTAACTCTGCAG9920.7509348836505124No Hit
GTGTTAGTTCAGATTAATTCACGACCCCAAGTAGTCTGCGAGTGTCTCAG9720.7357950674478811No Hit
GGCATGTGTCAGTTGGAAGATCAGCTGGAGAGAACTTTGGGCTATGACTG9140.6918896004602504No Hit
GTGTACGGGTGTTCGCTCATTAAAACATATTCTGTCTGGGTGTAAAACTA8380.6343582988902515No Hit
CCTCCTAACTCTGCAGACCACCTCAGTCAGTGGGTGGGTGAAGACAGAAG8300.628302372409199No Hit
AGCAGGGGCAATGGATGAACTGGCATGTGTCAGTTGGAAGATCAGCTGGA8090.6124055653964361No Hit
ACCAGAGACAACAACAGATACACCTCAACATGATTTCACATATCTCAGTG7750.586667877851963No Hit
GAACTGGCATGTGTCAGTTGGAAGATCAGCTGGAGAGAACTTTGGGCTAT7000.5298935670920956No Hit
TCTCAGTGTTAGTTCAGATTAATTCACGACCCCAAGTAGTCTGCGAGTGT6930.5245946314211746No Hit
GGCTATGACTGGTAACCGCATTATATTCATAGTTGGAGCCATTTTTTATG6560.4965859714463066No Hit
CCTCAACATGATTTCACATATCTCAGTGTTAGTTCAGATTAATTCACGAC6400.48447411848420163No Hit
CTACCAGAGACAACAACAGATACACCTCAACATGATTTCACATATCTCAG6350.4806891644335438No Hit
CAACAGATACACCTCAACATGATTTCACATATCTCAGTGTTAGTTCAGAT6280.4753902287626228No Hit
CAGATACACCTCAACATGATTTCACATATCTCAGTGTTAGTTCAGATTAA6260.4738762471423597No Hit
CGCCACTACCAGAGACAACAACAGATACACCTCAACATGATTTCACATAT6230.471605274711965No Hit
ATTCATAGTTGGAGCCATTTTTTATGTCCTCCTAACTCTGCAGACCACCT5900.4466245779776234No Hit
GGCAATGGATGAACTGGCATGTGTCAGTTGGAAGATCAGCTGGAGAGAAC5870.44435360554722864No Hit
GTCAGTGGGTGGGTGAAGACAGAAGCTGCAAGTTGTGTACGGGTGTTCGC5610.42467184448380796No Hit
ACTTTGGGCTATGACTGGTAACCGCATTATATTCATAGTTGGAGCCATTT5460.4133169823318345No Hit
GTCCCAGACCCACCCCCCTCCGTATTCATAACGGGTTAAGACACACGCCA5420.41028901909130827No Hit
ACCCCAAGTAGTCTGCGAGTGTCTCAGCCACAACCACTGGCTCACTTTTT5420.41028901909130827No Hit
GGAGAGAACTTTGGGCTATGACTGGTAACCGCATTATATTCATAGTTGGA5250.39742017531907164No Hit
GTGTTTTGATGACCAAGTGGTGGCCGACGTCCCAGACCCACCCCCCTCCG5240.39666318450894006No Hit
CCGCATTATATTCATAGTTGGAGCCATTTTTTATGTCCTCCTAACTCTGC5180.39212123964815065No Hit
AGCAGGCACTTTCCTTCATCGGTGTTTTGATGACCAAGTGGTGGCCGACG5090.3853083223569666No Hit
GTGTCAGTTGGAAGATCAGCTGGAGAGAACTTTGGGCTATGACTGGTAAC5090.3853083223569666No Hit
GCTCTATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACT4580.3467017910402568No Hit
GATTAATTCACGACCCCAAGTAGTCTGCGAGTGTCTCAGCCACAACCACT4570.3459448002301252No Hit
CTCCTAACTCTGCAGACCACCTCAGTCAGTGGGTGGGTGAAGACAGAAGC4560.3451878094199936No Hit
TCCTAACTCTGCAGACCACCTCAGTCAGTGGGTGGGTGAAGACAGAAGCT4540.34367382779973055No Hit
AACATATTCTGTCTGGGTGTAAAACTAGCCTCACCCAGGTCCGCTACGCG4470.33837489212880956No Hit
TCCGTATTCATAACGGGTTAAGACACACGCCACTACCAGAGACAACAACA4340.3285340115970992No Hit
CCGTATTCATAACGGGTTAAGACACACGCCACTACCAGAGACAACAACAG4250.32172109430591517No Hit
ACCTCAGTCAGTGGGTGGGTGAAGACAGAAGCTGCAAGTTGTGTACGGGT4240.3209641034957836No Hit
CCTCCGTATTCATAACGGGTTAAGACACACGCCACTACCAGAGACAACAA4230.320207112685652No Hit
CATTAAAACATATTCTGTCTGGGTGTAAAACTAGCCTCACCCAGGTCCGC4170.3156651678248626No Hit
GTTGGAAGATCAGCTGGAGAGAACTTTGGGCTATGACTGGTAACCGCATT4170.3156651678248626No Hit
GACAACAACAGATACACCTCAACATGATTTCACATATCTCAGTGTTAGTT4060.30733826891341537No Hit
CAACAACAGATACACCTCAACATGATTTCACATATCTCAGTGTTAGTTCA4010.30355331486275755No Hit
GGTGAAGACAGAAGCTGCAAGTTGTGTACGGGTGTTCGCTCATTAAAACA3930.29749738838170503No Hit
GGGCAATGGATGAACTGGCATGTGTCAGTTGGAAGATCAGCTGGAGAGAA3910.2959834067614419No Hit
CTGCAGACCACCTCAGTCAGTGGGTGGGTGAAGACAGAAGCTGCAAGTTG3870.2929554435209156No Hit
CCCAAGTAGTCTGCGAGTGTCTCAGCCACAACCACTGGCTCACTTTTTCG3850.29144146190065257No Hit
CATTATATTCATAGTTGGAGCCATTTTTTATGTCCTCCTAACTCTGCAGA3820.2891704894702578No Hit
ATTTCACATATCTCAGTGTTAGTTCAGATTAATTCACGACCCCAAGTAGT3810.2884134986601262No Hit
CATAACGGGTTAAGACACACGCCACTACCAGAGACAACAACAGATACACC3770.2853855354196No Hit
GGGTTAAGACACACGCCACTACCAGAGACAACAACAGATACACCTCAACA3750.2838715537993369No Hit
TCACATATCTCAGTGTTAGTTCAGATTAATTCACGACCCCAAGTAGTCTG3710.2808435905588106No Hit
CTTTCCTTCATCGGTGTTTTGATGACCAAGTGGTGGCCGACGTCCCAGAC3650.27630164569802124No Hit
GTGTGTGTGTGACCTTGCTGATCTTGTGGGTGTTTCTTGGTGGACAGGGG3650.27630164569802124No Hit
AGCGAAAGCAGGGGCAATGGATGAACTGGCATGTGTCAGTTGGAAGATCA3570.2702457192169687No Hit
TTTTTTATGTCCTCCTAACTCTGCAGACCACCTCAGTCAGTGGGTGGGTG3560.26948872840683713No Hit
CCACTACCAGAGACAACAACAGATACACCTCAACATGATTTCACATATCT3530.26721775597644243No Hit
ACCAAGTGGTGGCCGACGTCCCAGACCCACCCCCCTCCGTATTCATAACG3510.2657037743561793No Hit
TGGATGAACTGGCATGTGTCAGTTGGAAGATCAGCTGGAGAGAACTTTGG3480.2634328019257846No Hit
TATTCATAGTTGGAGCCATTTTTTATGTCCTCCTAACTCTGCAGACCACC3470.262675811115653No Hit
GTCTGCGAGTGTCTCAGCCACAACCACTGGCTCACTTTTTCGAACAAACA3460.2619188203055215No Hit
GCTGGAGAGAACTTTGGGCTATGACTGGTAACCGCATTATATTCATAGTT3450.26116182949538996No Hit
GGGGCAATGGATGAACTGGCATGTGTCAGTTGGAAGATCAGCTGGAGAGA3400.2573768754447321No Hit
ATCTCAGTGTTAGTTCAGATTAATTCACGACCCCAAGTAGTCTGCGAGTG3280.2482929857231533No Hit
GCGAAAGCAGGGGCAATGGATGAACTGGCATGTGTCAGTTGGAAGATCAG3270.24753599491302175No Hit
AAGCAGGACCGATCTGGCTGCGTGAGTCCCTCTGTGTGTGTGTGTGTTTC3260.24677900410289016No Hit
GATCTGGGATGCTGAATGTACCACTGCAGACTCTCTGCAGAAGAGTAATT3110.2354241419509167No Hit
CCTCAGTCAGTGGGTGGGTGAAGACAGAAGCTGCAAGTTGTGTACGGGTG3050.23088219709012733No Hit
GTTGTGTACGGGTGTTCGCTCATTAAAACATATTCTGTCTGGGTGTAAAA3030.2293682154698642No Hit
GTGGTGGCCGACGTCCCAGACCCACCCCCCTCCGTATTCATAACGGGTTA3010.22785423384960105No Hit
ACTGGTAACCGCATTATATTCATAGTTGGAGCCATTTTTTATGTCCTCCT2990.22634025222933793No Hit
GACCCACCCCCCTCCGTATTCATAACGGGTTAAGACACACGCCACTACCA2910.22028432574828538No Hit
GTGGGTGGGTGAAGACAGAAGCTGCAAGTTGTGTACGGGTGTTCGCTCAT2830.2142283992672329No Hit
CAAGTAGTCTGCGAGTGTCTCAGCCACAACCACTGGCTCACTTTTTCGAA2760.20892946359631193No Hit
GGTAACCGCATTATATTCATAGTTGGAGCCATTTTTTATGTCCTCCTAAC2700.20438751873552258No Hit
AAGCAGGGGCAATGGATGAACTGGCATGTGTCAGTTGGAAGATCAGCTGG2690.203630527925391No Hit
CTGGTAACCGCATTATATTCATAGTTGGAGCCATTTTTTATGTCCTCCTA2670.20211654630512785No Hit
GGCACTTTCCTTCATCGGTGTTTTGATGACCAAGTGGTGGCCGACGTCCC2660.20135955549499632No Hit
TAGTTGGAGCCATTTTTTATGTCCTCCTAACTCTGCAGACCACCTCAGTC2650.20060256468486473No Hit
ACCCCCCTCCGTATTCATAACGGGTTAAGACACACGCCACTACCAGAGAC2640.19984557387473317No Hit
AGATACACCTCAACATGATTTCACATATCTCAGTGTTAGTTCAGATTAAT2630.1990885830646016No Hit
ACATATCTCAGTGTTAGTTCAGATTAATTCACGACCCCAAGTAGTCTGCG2570.1945466382038122No Hit
AACTGGCATGTGTCAGTTGGAAGATCAGCTGGAGAGAACTTTGGGCTATG2550.19303265658354907No Hit
GTCTCAGCCACAACCACTGGCTCACTTTTTCGAACAAACATGCAAATGAT2530.19151867496328595No Hit
CCTAACTCTGCAGACCACCTCAGTCAGTGGGTGGGTGAAGACAGAAGCTG2530.19151867496328595No Hit
GCCTTAGCCAGCCGATGCATCTGAGCTCCTGACAGCGGCAAACAACCCCC2480.1877337209126281No Hit
CAACATGATTTCACATATCTCAGTGTTAGTTCAGATTAATTCACGACCCC2450.1854627484822334No Hit
GTGTAAAACTAGCCTCACCCAGGTCCGCTACGCGTGAAGACAGATTCAGG2450.1854627484822334No Hit
TTACAGGACAAGGTAACATTCAGTCCTTCCTCTGTAAAGACTTCGGTTCT2440.18470575767210187No Hit
TTCCTTCATCGGTGTTTTGATGACCAAGTGGTGGCCGACGTCCCAGACCC2410.18243478524170717No Hit
CCCCCCTCCGTATTCATAACGGGTTAAGACACACGCCACTACCAGAGACA2370.1794068220011809No Hit
CTTGTAAATATGGTGGTGAAAGACCTGCAGTCTTTTTCCATTGTGGACAG2320.1756218679505231No Hit
GTTGGAGCCATTTTTTATGTCCTCCTAACTCTGCAGACCACCTCAGTCAG2270.17183691389986527No Hit
CACCTCAACATGATTTCACATATCTCAGTGTTAGTTCAGATTAATTCACG2270.17183691389986527No Hit
ACTCTGCAGACCACCTCAGTCAGTGGGTGGGTGAAGACAGAAGCTGCAAG2260.1710799230897337No Hit
AGGTAGGAGAGGGGGATGATTAAAGATGAAAAAAGTGTTTGTGAAAGAGA2260.1710799230897337No Hit
CTATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCAT2250.17032293227960213No Hit
TAACTCTGCAGACCACCTCAGTCAGTGGGTGGGTGAAGACAGAAGCTGCA2220.16805195984920743No Hit
GTTCAGATTAATTCACGACCCCAAGTAGTCTGCGAGTGTCTCAGCCACAA2220.16805195984920743No Hit
AAAGCAGGCACTTTCCTTCATCGGTGTTTTGATGACCAAGTGGTGGCCGA2170.1642670057985496No Hit
CAGCTGGAGAGAACTTTGGGCTATGACTGGTAACCGCATTATATTCATAG2130.16123904255802335No Hit
GTAAAGAAGAGAGAGAGGTAAAGAAGAGGGAGAGGTAAAGAGAGCAGGAG2100.15896807012762865No Hit
GTTTTGATGACCAAGTGGTGGCCGACGTCCCAGACCCACCCCCCTCCGTA2080.1574540885073655No Hit
GGGCTATGACTGGTAACCGCATTATATTCATAGTTGGAGCCATTTTTTAT2040.15442612526683927No Hit
ATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCATCT2010.15215515283644457No Hit
ATGTACCACTGCAGACTCTCTGCAGAAGAGTAATTACAGGACAAGGTAAC1980.14988418040604987No Hit
GTTAAGACACACGCCACTACCAGAGACAACAACAGATACACCTCAACATG1930.14609922635539205No Hit
ATGTACGGCTTGGTCCCGCCGCAGATCTGAAAAAGAAAAAGGGTCAAAGA1930.14609922635539205No Hit
TTTGATGACCAAGTGGTGGCCGACGTCCCAGACCCACCCCCCTCCGTATT1900.14382825392499737No Hit
GATACACCTCAACATGATTTCACATATCTCAGTGTTAGTTCAGATTAATT1880.1423142723047342No Hit
GTGCTCGGCCTGCCTCTGTGATTGATAGCTGGGGAGCCGGAGCCCTGTGT1850.14004329987433953No Hit
GTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCATCTCATGT1830.13852931825407638No Hit
GAATGTACCACTGCAGACTCTCTGCAGAAGAGTAATTACAGGACAAGGTA1810.13701533663381327No Hit
GAACTGGGGAGCTGATCTGGGATGCTGAATGTACCACTGCAGACTCTCTG1790.13550135501355012No Hit
GTGTGTGCATGTGCGTGTGTGGGTACATGTGCCTTTGGCCCAGTGTGTGT1770.133987373393287No Hit
ACTGGCATGTGTCAGTTGGAAGATCAGCTGGAGAGAACTTTGGGCTATGA1770.133987373393287No Hit
GTATTCATAACGGGTTAAGACACACGCCACTACCAGAGACAACAACAGAT1770.133987373393287No Hit
CTCTGCAGAAGAGTAATTACAGGACAAGGTAACATTCAGTCCTTCCTCTG1730.13095941015276075No Hit
ACAACAGATACACCTCAACATGATTTCACATATCTCAGTGTTAGTTCAGA1690.1279314469122345No Hit
ACACACGCCACTACCAGAGACAACAACAGATACACCTCAACATGATTTCA1690.1279314469122345No Hit
GCATACTGAGTGTGCTCCAAGTACCTCTCTCCTGCTCTCTTTACCTCTCT1670.12641746529197137No Hit
ATGGATGAACTGGCATGTGTCAGTTGGAAGATCAGCTGGAGAGAACTTTG1670.12641746529197137No Hit
ATTTTTTATGTCCTCCTAACTCTGCAGACCACCTCAGTCAGTGGGTGGGT1660.12566047448183978No Hit
ATGACTGGTAACCGCATTATATTCATAGTTGGAGCCATTTTTTATGTCCT1660.12566047448183978No Hit
ATACACCTCAACATGATTTCACATATCTCAGTGTTAGTTCAGATTAATTC1650.12490348367170823No Hit
CTGCATACTGAGTGTGCTCCAAGTACCTCTCTCCTGCTCTCTTTACCTCT1640.12414649286157665No Hit
AAGTAGGAGTGACTCACAGTGAGCTCTATACTGTACTCTAGTAGCCACCT1630.12338950205144508No Hit
ATTATATTCATAGTTGGAGCCATTTTTTATGTCCTCCTAACTCTGCAGAC1630.12338950205144508No Hit
AAAGCAGGGGCAATGGATGAACTGGCATGTGTCAGTTGGAAGATCAGCTG1620.12263251124131354No Hit
CTGCAGACTCTCTGCAGAAGAGTAATTACAGGACAAGGTAACATTCAGTC1610.12187552043118197No Hit
GAACTTTGGGCTATGACTGGTAACCGCATTATATTCATAGTTGGAGCCAT1600.12111852962105041No Hit
GAATATATTCTGAGTGTGCGCTGGGGGAAAAGATGGATGGACCGAGAAGG1590.12036153881091884No Hit
AGCGAAAGCAGGCACTTTCCTTCATCGGTGTTTTGATGACCAAGTGGTGG1590.12036153881091884No Hit
TGATTTCACATATCTCAGTGTTAGTTCAGATTAATTCACGACCCCAAGTA1570.1188475571906557No Hit
GACACACGCCACTACCAGAGACAACAACAGATACACCTCAACATGATTTC1540.116576584760261No Hit
ATTTAATTCCTCCTCAGAGCCGCCAGGCCATTTGTAAAAAGAGAAAGTCT1540.116576584760261No Hit
GGTGTTCGCTCATTAAAACATATTCTGTCTGGGTGTAAAACTAGCCTCAC1520.11506260313999789No Hit
CCACCTCAGTCAGTGGGTGGGTGAAGACAGAAGCTGCAAGTTGTGTACGG1520.11506260313999789No Hit
GTGTGTGACCTTGCTGATCTTGTGGGTGTTTCTTGGTGGACAGGGGAGTA1520.11506260313999789No Hit
GCACTTTCCTTCATCGGTGTTTTGATGACCAAGTGGTGGCCGACGTCCCA1510.11430561232986632No Hit
CTTAAGAACATTGTGGTCCAAAAATATGAAGAGGAGAAGACCAAGGCCAG1510.11430561232986632No Hit
GTACGGGTGTTCGCTCATTAAAACATATTCTGTCTGGGTGTAAAACTAGC1500.11354862151973476No Hit
GATGAACTGGCATGTGTCAGTTGGAAGATCAGCTGGAGAGAACTTTGGGC1500.11354862151973476No Hit
CTTTATAATATACTTTCTTAAAGTGTCTGGTGTGTGTAAAGAGGAGGCAC1470.11127764908934006No Hit
AAACTAGCCTCACCCAGGTCCGCTACGCGTGAAGACAGATTCAGGTTTTG1450.10976366746907693No Hit
GATCAGCTGGAGAGAACTTTGGGCTATGACTGGTAACCGCATTATATTCA1440.10900667665894535No Hit
GTGTGTGTGTGTGTTTCTGAAAGTGCGTTTGTGTGTGAGTGTGTGTCTAT1430.10824968584881378No Hit
CAGTCAGTGGGTGGGTGAAGACAGAAGCTGCAAGTTGTGTACGGGTGTTC1420.10749269503868224No Hit
TGACTGGTAACCGCATTATATTCATAGTTGGAGCCATTTTTTATGTCCTC1400.10597871341841911No Hit
GTTCAGAGCATGAACAAAGCAGATGACATGGCGAAGATTTAAACTTCGCA1380.10446473179815596No Hit
ACATGATTTCACATATCTCAGTGTTAGTTCAGATTAATTCACGACCCCAA1380.10446473179815596No Hit
ACCCACCCCCCTCCGTATTCATAACGGGTTAAGACACACGCCACTACCAG1370.1037077409880244No Hit
TTAAAACATATTCTGTCTGGGTGTAAAACTAGCCTCACCCAGGTCCGCTA1370.1037077409880244No Hit
CTCTGTGTGTGTGTGTGTTTCTGAAAGTGCGTTTGTGTGTGAGTGTGTGT1370.1037077409880244No Hit
GACCACCTCAGTCAGTGGGTGGGTGAAGACAGAAGCTGCAAGTTGTGTAC1360.10295075017789283No Hit
GCCCTCAGGGAGCCGCCGGTAAACACACGGCCTTAGCCAGCCGATGCATC1350.10219375936776129No Hit
ATAATATACTTTCTTAAAGTGTCTGGTGTGTGTAAAGAGGAGGCACACAA1350.10219375936776129No Hit
CAGTGTTAGTTCAGATTAATTCACGACCCCAAGTAGTCTGCGAGTGTCTC1340.1014367685576297No Hit
GCCACAACCACTGGCTCACTTTTTCGAACAAACATGCAAATGATTTCAAA1340.1014367685576297No Hit
TACCTACACTCTGCCATCCAGGCGGGCTCTTAAGAACATTGTGGTCCAAA1330.10067977774749816No Hit
CTCTTGTGTGATACACGGGGATGGTGCTCGGCCTGCCTCTGTGATTGATA1330.10067977774749816No Hit
GTGTGATACACGGGGATGGTGCTCGGCCTGCCTCTGTGATTGATAGCTGG1330.10067977774749816No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTTT100.007070252145.09373145
GTTCACC100.0070943544144.928737
TGGTTCA100.0071024005144.873815
ATTGGTT100.0071024005144.873813
TTGGTTC100.0071024005144.873814
GTATTGG100.007118511144.764111
AGCGAAA1800.0120.636761
ACCGATC458.367351E-11112.722358
GACCGAT458.367351E-11112.722357
CCGATCT458.367351E-11112.722359
CAGGACC458.367351E-11112.6796344
AGGACCG501.9463187E-10101.411675
GCGAAAG2400.090.5461352
CGAAAGC2400.090.5461353
CAGGACG259.386877E-486.957249
GCGTTAT259.4293786E-486.858461
TCAGCCC850.085.34925145
GCAGGAC755.638867E-1177.266043
GACTTCT300.001944530372.382051
GCAGGGA554.063053E-665.851744
Produced by FastQC (version 0.11.5)