FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_C09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_C09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613630
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA44700.27701517696126127No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG42510.26344329245242093No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT33240.20599517857253524No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC33180.2056233461202382No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC25890.16044570316615334No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG23620.14637804205425037No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG21720.1346033477315122No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20800.1289019167962916No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20440.12667092208250963No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC18150.11247931681984098No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG17310.10727366248768305No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT17130.10615816513079206No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC16600.10287364513550194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3400.057.5354271
GTAGAAA14000.044.4965862
AGCTATC6550.044.264729
AGTAGAA14700.041.8940281
TTATACT19700.039.3569954
TAAGATG15100.039.354575
GTCTAGT12400.039.1474651
CCCTTAT18700.039.131841
CGAAAGC5100.038.361713
GCGAAAG5350.037.9105872
CAATTCG5200.037.6356549
CCTTATA19800.037.315672
GAGCTAT8150.036.4618238
ATTAAGA16100.035.105333
CCATCGG1102.4400698E-432.9470379
TTAAGAT18200.031.850994
TGGTGCG1601.8823139E-631.7095578
ACCGTGC22350.030.8076258
GCATTAA20850.030.5792711
TCATTCG950.004734852430.5174718
Produced by FastQC (version 0.11.5)