FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534662
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT47140.8816785183910584No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC32150.6013144753133756No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC26940.5038697345238674No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG26820.5016253259068346No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG19220.3594794468280895No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG17600.3291799304981465No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC16790.314030172333175No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC16730.31290796802465853No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA15270.285600996517426No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA15060.2816732814376185No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT14190.2654013189641306No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT13810.2582940250101934No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT12040.22518899790895933No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC11970.2238797595490235No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC11820.22107424877773246No Hit
TGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGA11280.2109744100010848No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT10030.1875951535736596No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG9940.18591184711088501No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA9540.17843048505410897No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG9370.17525090617997913No Hit
GTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTAT9280.1735675997172045No Hit
TTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAG9020.16870471438030007No Hit
GTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAG9010.16851768032888068No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA8650.16178445447778222No Hit
GATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAA8620.161223352323524No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA8490.1587919096550718No Hit
CTTTTGGGCAGCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGG7990.14944020708410172No Hit
GTAGAAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCC7410.13859223210177643No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG7380.13803112994751823No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA7350.13747002779326006No Hit
GAATGGAACATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATC7280.13616078943332424No Hit
GGGAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTA7080.1324201084049362No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT6870.12849239332512877No Hit
ATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACTCG6640.12419061014248255No Hit
GTTCCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAA6460.12082399721693332No Hit
GAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAA6100.11409077136583486No Hit
GTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCACCCGCATAAT5750.1075445795661558No Hit
TGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTGG5560.1039909325891872No Hit
CATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTA5540.10361686448634838No Hit
GAGCAATACTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATT5400.10099838776647675No Hit
ATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTT5360.10025025156079916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC100.0071067424144.885284
GCATTAA10550.073.4521
AGATGTA11600.064.366237
AGCGAAA1051.6370905E-1162.076261
CATTAAG13450.058.147782
TTAAGAT12750.057.385934
ATTAAGA13350.055.892073
TAAGATG13900.053.6855355
GTAGAAA6100.048.6772881
GTCTAGT12450.043.046191
GCGAAAG1557.4578566E-1042.051661
AAGATGT18350.041.065096
ACGACAC750.001488545938.6360743
TAGTTAA13700.038.072044
GATTAGC5750.035.2995388
CGAAAGC1905.458787E-934.314933
CAACCGT850.002748980734.0970346
TATAGGG1301.662326E-533.435064
AAGGATT6550.033.1829385
CCGGAAT1102.4446347E-432.9315535
Produced by FastQC (version 0.11.5)