FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD-S4_R1_B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD-S4_R1_B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461846
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA9000.19487015152236892No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT8460.18317794243102678No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAAGATCAAAT7030.15221524057802818No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG5390.11670556852284096No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA4830.10458031465033799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2150.063.992751
GCGAAAG2650.054.6275332
TATAGTA805.93338E-754.2861142
TATAGAC2700.053.6159132
ATCGCAG450.00960739148.36955145
GTATAGT951.934577E-645.734431
GTATGAG803.7581092E-545.258031
CAGCTCG3600.042.2728849
CGAAAGC3450.041.987563
ATAGACC2109.094947E-1237.938763
TTACATC1553.490277E-837.3865244
TATACAG4200.036.2221225
TATAAAG3700.035.2278751
CTACACC1501.1399297E-633.8036464
TTACACT1751.0149779E-733.1137774
GATCGCT1102.4404812E-432.9399077
TACATAC4900.032.5365646
CGACGGT900.00363411732.2079126
TATATAG900.003655392732.1695482
TACAGCT5200.030.6594547
Produced by FastQC (version 0.11.5)